TY - JOUR
T1 - Proteomic profiling of rapid non-genomic and concomitant genomic effects of acute restraint stress on rat thymocytes
AU - Billing, Anja M.
AU - Revets, Dominique
AU - Hoffmann, Céline
AU - Turner, Jonathan D.
AU - Vernocchi, Sara
AU - Muller, Claude P.
N1 - Funding Information:
AMB was supported by CRP-Santé and the Ministry of Culture, Higher Education and Research, Luxembourg . We greatly acknowledge the excellent services of Jenny Renaut and Sébastien Planchon from the CRP-Gabriel Lippmann for protein identification. We also thank Slavena Trifonova and Oliver Hunewald for their contributions.
PY - 2012/4/3
Y1 - 2012/4/3
N2 - In order to investigate rapid non-genomic effects of acute stress, rats were restrained for 15. min which was sufficient to activate the hypothalamus-pituitary-adrenal (HPA) axis but too short to induce massive genomic effects of cortisol. Subcellular fractions of thymocytes (cytosol, nucleus, membrane) were investigated using quantitative 2D DIGE with MALDI-TOF/TOF mass spectrometry. In total, 108 proteins with differential subcellular localizations were identified. The specificity of the changes induced by psychological stress was reflected by the prominent modulation of proteins involved in the HPA and sympathoadrenal medullar (SAM) axis such as HMGB1 and NHERF1. Intracellular trafficking was characterized by a dominant protein exodus from the cytosol. Real translocation was observed for 9 proteins with 6 that shuttled from the cytosol to the nucleus (HYOU1, HNRPF, HNRPC, STRAP, PSA1, PPA1) and 3 from the nucleus to the cytosol (HMGB1, NHERF1, PSMA1). Proteins showing subcellular reshuffling were largely involved in transcription and translation processes (39 of 108) with a significant enrichment of RNA splicing factors. Bioinformatics analysis revealed significant enrichment for protein kinase A and 14-3-3 signaling, probably reflecting real non-genomic effects. This is the first study investigating rapid effects of stress-induced HPA activation in vivo at the proteome level.
AB - In order to investigate rapid non-genomic effects of acute stress, rats were restrained for 15. min which was sufficient to activate the hypothalamus-pituitary-adrenal (HPA) axis but too short to induce massive genomic effects of cortisol. Subcellular fractions of thymocytes (cytosol, nucleus, membrane) were investigated using quantitative 2D DIGE with MALDI-TOF/TOF mass spectrometry. In total, 108 proteins with differential subcellular localizations were identified. The specificity of the changes induced by psychological stress was reflected by the prominent modulation of proteins involved in the HPA and sympathoadrenal medullar (SAM) axis such as HMGB1 and NHERF1. Intracellular trafficking was characterized by a dominant protein exodus from the cytosol. Real translocation was observed for 9 proteins with 6 that shuttled from the cytosol to the nucleus (HYOU1, HNRPF, HNRPC, STRAP, PSA1, PPA1) and 3 from the nucleus to the cytosol (HMGB1, NHERF1, PSMA1). Proteins showing subcellular reshuffling were largely involved in transcription and translation processes (39 of 108) with a significant enrichment of RNA splicing factors. Bioinformatics analysis revealed significant enrichment for protein kinase A and 14-3-3 signaling, probably reflecting real non-genomic effects. This is the first study investigating rapid effects of stress-induced HPA activation in vivo at the proteome level.
KW - Acute stress
KW - Alternative splicing
KW - Glucocorticoid receptor
KW - HMGB1
KW - NHERF1
KW - Non-genomic effects
UR - http://www.scopus.com/inward/record.url?scp=84858753519&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2012.01.008
DO - 10.1016/j.jprot.2012.01.008
M3 - Article
C2 - 22270012
AN - SCOPUS:84858753519
SN - 1874-3919
VL - 75
SP - 2064
EP - 2079
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 7
ER -