TY - JOUR
T1 - Proteome profiling of virus-host interactions of wild type and attenuated measles virus strains
AU - Billing, Anja M.
AU - Kessler, Julia R.
AU - Revets, Dominique
AU - Sausy, Aurélie
AU - Schmitz, Stephanie
AU - Barra, Claire
AU - Muller, Claude P.
N1 - Funding Information:
This work was supported by CRP-Santé with a postdoctoral grant to AMB, CB and by Fonds National de la Recherche Luxembourg with a Ph.D. grant ( BFR04/036 ) to JRK. We would like to thank JM Huebschen for her help in the sequencing analysis.
PY - 2014/8/28
Y1 - 2014/8/28
N2 - Quantitative gel-based proteomics (2D DIGE coupled to MALDI-TOF/TOF MS) has been used to investigate the effects of different measles virus (MV) strains on the host cell proteome. A549/hSLAM cells were infected either with wild type MV strains, an attenuated vaccine or a multiple passaged Vero cell adapted strain. By including interferon beta treatment as a control it was possible to distinguish between the classical antiviral response and changes induced specifically by the different strains. Of 38 differentially expressed proteins in total (p-value ≤. 0.05, fold change ≥. 2), 18 proteins were uniquely modulated following MV infection with up to 9 proteins specific per individual strain. Interestingly, wt strains displayed distinct protein patterns particularly during the late phase of infection. Proteins were grouped into cytoskeleton, metabolism, transcription/translation, immune response and mitochondrial proteins. Bioinformatics analysis revealed mostly changes in proteins regulating cell death and apoptosis. Surprisingly, wt strains affected the cytokeratin system much stronger than the vaccine strain. To our knowledge, this is the first study on the MV-host proteome addressing interstrain differences.
AB - Quantitative gel-based proteomics (2D DIGE coupled to MALDI-TOF/TOF MS) has been used to investigate the effects of different measles virus (MV) strains on the host cell proteome. A549/hSLAM cells were infected either with wild type MV strains, an attenuated vaccine or a multiple passaged Vero cell adapted strain. By including interferon beta treatment as a control it was possible to distinguish between the classical antiviral response and changes induced specifically by the different strains. Of 38 differentially expressed proteins in total (p-value ≤. 0.05, fold change ≥. 2), 18 proteins were uniquely modulated following MV infection with up to 9 proteins specific per individual strain. Interestingly, wt strains displayed distinct protein patterns particularly during the late phase of infection. Proteins were grouped into cytoskeleton, metabolism, transcription/translation, immune response and mitochondrial proteins. Bioinformatics analysis revealed mostly changes in proteins regulating cell death and apoptosis. Surprisingly, wt strains affected the cytokeratin system much stronger than the vaccine strain. To our knowledge, this is the first study on the MV-host proteome addressing interstrain differences.
KW - Interferon beta
KW - Interstrain differences
KW - Measles virus
KW - Proteomics
KW - Virus-host interaction
UR - http://www.scopus.com/inward/record.url?scp=84902982358&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2014.05.029
DO - 10.1016/j.jprot.2014.05.029
M3 - Article
C2 - 24914991
AN - SCOPUS:84902982358
SN - 1874-3919
VL - 108
SP - 325
EP - 336
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -