Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

Luciana Bertholim; André Zelanis; Ana K. Oliveira; Solange M.T. Serrano

doi:10.1007/s00726-016-2218-z

Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

Luciana Bertholim, André Zelanis, Ana K. Oliveira, Solange M.T. Serrano^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

10 Citations (Scopus)

Abstract

The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6′ sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1′ position, followed by hydrophobic residues in P2′. PICS confirmed existing data on prime site specificity of SVMPs.

Original language	English
Pages (from-to)	1331-1335
Number of pages	5
Journal	Amino Acids
Volume	48
Issue number	5
DOIs	https://doi.org/10.1007/s00726-016-2218-z
Publication status	Published - 1 May 2016
Externally published	Yes

Keywords

HF3
Metalloproteinase
Peptide bond specificity
Proteome derived peptide library
Proteomic identification of protease cleavage sites
Snake venom

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10.1007/s00726-016-2218-z

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title = "Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase",

abstract = "The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6′ sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1′ position, followed by hydrophobic residues in P2′. PICS confirmed existing data on prime site specificity of SVMPs.",

keywords = "HF3, Metalloproteinase, Peptide bond specificity, Proteome derived peptide library, Proteomic identification of protease cleavage sites, Snake venom",

author = "Luciana Bertholim and Andr{\'e} Zelanis and Oliveira, \{Ana K.\} and Serrano, \{Solange M.T.\}",

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doi = "10.1007/s00726-016-2218-z",

language = "English",

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T1 - Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

AU - Bertholim, Luciana

AU - Zelanis, André

AU - Oliveira, Ana K.

AU - Serrano, Solange M.T.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6′ sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1′ position, followed by hydrophobic residues in P2′. PICS confirmed existing data on prime site specificity of SVMPs.

AB - The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6′ sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1′ position, followed by hydrophobic residues in P2′. PICS confirmed existing data on prime site specificity of SVMPs.

KW - HF3

KW - Metalloproteinase

KW - Peptide bond specificity

KW - Proteome derived peptide library

KW - Proteomic identification of protease cleavage sites

KW - Snake venom

UR - https://www.scopus.com/pages/publications/84961595168

U2 - 10.1007/s00726-016-2218-z

DO - 10.1007/s00726-016-2218-z

M3 - Article

C2 - 27020778

AN - SCOPUS:84961595168

SN - 0939-4451

VL - 48

SP - 1331

EP - 1335

JO - Amino Acids

JF - Amino Acids

IS - 5

ER -

Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

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Keywords

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