Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

Luciana Bertholim, André Zelanis, Ana K. Oliveira, Solange M.T. Serrano*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6′ sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1′ position, followed by hydrophobic residues in P2′. PICS confirmed existing data on prime site specificity of SVMPs.

Original languageEnglish
Pages (from-to)1331-1335
Number of pages5
JournalAmino Acids
Volume48
Issue number5
DOIs
Publication statusPublished - 1 May 2016
Externally publishedYes

Keywords

  • HF3
  • Metalloproteinase
  • Peptide bond specificity
  • Proteome derived peptide library
  • Proteomic identification of protease cleavage sites
  • Snake venom

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