Abstract
The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6′ sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1′ position, followed by hydrophobic residues in P2′. PICS confirmed existing data on prime site specificity of SVMPs.
Original language | English |
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Pages (from-to) | 1331-1335 |
Number of pages | 5 |
Journal | Amino Acids |
Volume | 48 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 May 2016 |
Externally published | Yes |
Keywords
- HF3
- Metalloproteinase
- Peptide bond specificity
- Proteome derived peptide library
- Proteomic identification of protease cleavage sites
- Snake venom