TY - JOUR
T1 - Protective effect of Dammarane Sapogenins against chemotherapy-induced myelosuppression in mice
AU - Yang, Yanyan
AU - Xu, Shuping
AU - Xu, Qiuxia
AU - Liu, Xinmin
AU - Gao, Yue
AU - Steinmetz, Andre
AU - Wang, Ning
AU - Wang, Tianshan
AU - Qiu, Guosong
N1 - Funding Information:
This work was supported by a grant from the Ministry of Science and Technology of China (Grant No. 2008015) and the European Commission (IRSES project: Traditional Chinese medicine in the post-genomic era: identifying lead therapeutic compounds against cancer [230232])
PY - 2011/6
Y1 - 2011/6
N2 - Chemotherapy is the most common way to treat malignancies, but myelosuppression, one of its common side-effects, is a formidable problem. The present study described the protective role of Dammarane Sapogenins (DS), an active fraction from oriental ginseng, on myelosuppression induced by cyclophosphamide (CP) in mice. DS was orally administered at different dosages (37.5, 75, and 150 mg/kg) for 10 d after CP administration (200 mg/kg intraperitoneally). The results showed that DS increased the number of white blood cells (WBC) on day 3 and day 7 (P< 0.05), such that WBC levels were increased by 105.7±29.5% at 75 mg/kg of DS on day 3 (P< 0.05, compared with the CP group). Similar results were observed in red blood cells and platelets in DS-treated groups. The colony-forming assay demonstrated that the depressed numbers of CFU-GM (colony-forming unit-granulocyte and macrophage), CFU-E (colony-forming unit-erythroid), BFU-E (burst-forming unit-erythroid), CFU-Meg (colony-forming unit-megakaryocyte) and CFU-GEMM (colony-forming unitgranulocyte, -erythrocyte, -monocyte and -megakaryocyte) induced by CP were significantly reversed after DS treatment. Moreover, the ameliorative effect of DS on myelosuppression was also observed in the femur by hematoxylin/eosin staining. In DS-treated groups, ConA-induced splenocyte proliferation was enhanced significantly at all the doses (37.5, 75, 150 mg/kg) on day 3 at the rate of 50.3±8.0%, 77.6±8.5% and 44.5±8.4%, respectively, while lipopolysaccharideinduced proliferation was increased mainly on day 7 (P< 0.01), with an increased rate of 39.8±5.6%, 34.9±6.6% and 38.3±7.3%, respectively. The thymus index was also markedly increased by 70.4% and 36.6% at 75 mg/kg on days 3 and 7, respectively, as compared with the CP group. In summary, DS has a protective function against CP-induced myelosuppression. Its mechanism might be related to stimulating hematopoiesis recovery, as well as enhancing the immunological function.
AB - Chemotherapy is the most common way to treat malignancies, but myelosuppression, one of its common side-effects, is a formidable problem. The present study described the protective role of Dammarane Sapogenins (DS), an active fraction from oriental ginseng, on myelosuppression induced by cyclophosphamide (CP) in mice. DS was orally administered at different dosages (37.5, 75, and 150 mg/kg) for 10 d after CP administration (200 mg/kg intraperitoneally). The results showed that DS increased the number of white blood cells (WBC) on day 3 and day 7 (P< 0.05), such that WBC levels were increased by 105.7±29.5% at 75 mg/kg of DS on day 3 (P< 0.05, compared with the CP group). Similar results were observed in red blood cells and platelets in DS-treated groups. The colony-forming assay demonstrated that the depressed numbers of CFU-GM (colony-forming unit-granulocyte and macrophage), CFU-E (colony-forming unit-erythroid), BFU-E (burst-forming unit-erythroid), CFU-Meg (colony-forming unit-megakaryocyte) and CFU-GEMM (colony-forming unitgranulocyte, -erythrocyte, -monocyte and -megakaryocyte) induced by CP were significantly reversed after DS treatment. Moreover, the ameliorative effect of DS on myelosuppression was also observed in the femur by hematoxylin/eosin staining. In DS-treated groups, ConA-induced splenocyte proliferation was enhanced significantly at all the doses (37.5, 75, 150 mg/kg) on day 3 at the rate of 50.3±8.0%, 77.6±8.5% and 44.5±8.4%, respectively, while lipopolysaccharideinduced proliferation was increased mainly on day 7 (P< 0.01), with an increased rate of 39.8±5.6%, 34.9±6.6% and 38.3±7.3%, respectively. The thymus index was also markedly increased by 70.4% and 36.6% at 75 mg/kg on days 3 and 7, respectively, as compared with the CP group. In summary, DS has a protective function against CP-induced myelosuppression. Its mechanism might be related to stimulating hematopoiesis recovery, as well as enhancing the immunological function.
KW - Cyclophosphamide
KW - Dammarane sapogenins
KW - Hematopoiesis
KW - Immunoregulation
KW - Myelosuppression
KW - Splenocyte proliferation
UR - http://www.scopus.com/inward/record.url?scp=79958844512&partnerID=8YFLogxK
U2 - 10.1258/ebm.2011.010369
DO - 10.1258/ebm.2011.010369
M3 - Article
C2 - 21652604
AN - SCOPUS:79958844512
SN - 1535-3702
VL - 236
SP - 729
EP - 735
JO - Experimental Biology and Medicine
JF - Experimental Biology and Medicine
IS - 6
ER -