Protective effect of Dammarane Sapogenins against chemotherapy-induced myelosuppression in mice

Yanyan Yang, Shuping Xu, Qiuxia Xu, Xinmin Liu*, Yue Gao, Andre Steinmetz, Ning Wang, Tianshan Wang, Guosong Qiu

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)

Abstract

Chemotherapy is the most common way to treat malignancies, but myelosuppression, one of its common side-effects, is a formidable problem. The present study described the protective role of Dammarane Sapogenins (DS), an active fraction from oriental ginseng, on myelosuppression induced by cyclophosphamide (CP) in mice. DS was orally administered at different dosages (37.5, 75, and 150 mg/kg) for 10 d after CP administration (200 mg/kg intraperitoneally). The results showed that DS increased the number of white blood cells (WBC) on day 3 and day 7 (P< 0.05), such that WBC levels were increased by 105.7±29.5% at 75 mg/kg of DS on day 3 (P< 0.05, compared with the CP group). Similar results were observed in red blood cells and platelets in DS-treated groups. The colony-forming assay demonstrated that the depressed numbers of CFU-GM (colony-forming unit-granulocyte and macrophage), CFU-E (colony-forming unit-erythroid), BFU-E (burst-forming unit-erythroid), CFU-Meg (colony-forming unit-megakaryocyte) and CFU-GEMM (colony-forming unitgranulocyte, -erythrocyte, -monocyte and -megakaryocyte) induced by CP were significantly reversed after DS treatment. Moreover, the ameliorative effect of DS on myelosuppression was also observed in the femur by hematoxylin/eosin staining. In DS-treated groups, ConA-induced splenocyte proliferation was enhanced significantly at all the doses (37.5, 75, 150 mg/kg) on day 3 at the rate of 50.3±8.0%, 77.6±8.5% and 44.5±8.4%, respectively, while lipopolysaccharideinduced proliferation was increased mainly on day 7 (P< 0.01), with an increased rate of 39.8±5.6%, 34.9±6.6% and 38.3±7.3%, respectively. The thymus index was also markedly increased by 70.4% and 36.6% at 75 mg/kg on days 3 and 7, respectively, as compared with the CP group. In summary, DS has a protective function against CP-induced myelosuppression. Its mechanism might be related to stimulating hematopoiesis recovery, as well as enhancing the immunological function.

Original languageEnglish
Pages (from-to)729-735
Number of pages7
JournalExperimental Biology and Medicine
Volume236
Issue number6
DOIs
Publication statusPublished - Jun 2011

Keywords

  • Cyclophosphamide
  • Dammarane sapogenins
  • Hematopoiesis
  • Immunoregulation
  • Myelosuppression
  • Splenocyte proliferation

Fingerprint

Dive into the research topics of 'Protective effect of Dammarane Sapogenins against chemotherapy-induced myelosuppression in mice'. Together they form a unique fingerprint.

Cite this