TY - JOUR
T1 - Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1
AU - Melchior, Chantal
AU - Kreis, Stephanie
AU - Janji, Bassam
AU - Kieffer, Nelly
N1 - Funding Information:
We thank Dr. C. Muller (Laboratoire National de Santé, Luxembourg) for access to the automated DNA sequencer. This work was supported by grants from the Centre de Recherche Public-Santé (CRP-Santé, Luxembourg), the Centre National de la Recherche Scientifique (CNRS, France), the Fondation Luxembourgeoise contre le Cancer, and the Fondation “Aide aux enfants atteints d'un cancer”, Luxembourg.
PY - 2002/5/3
Y1 - 2002/5/3
N2 - Integrin-linked kinase (ILK)-1 is a 59-kDa serine-threonine protein kinase, which associates with the cytoplasmic domain of β1, β2 and β3 integrins and acts as a receptor proximal kinase regulating integrin-mediated signal transduction. We have recently identified an isoform of ILK (ILK-2), which is expressed, in a TGF-β1-dependent manner, in a highly invasive tumor cell line but not in normal adult tissues. In contrast, ILK-1 is ubiquitously expressed in normal tissues and is up-regulated in various tumors independent of TGF-β1. Here, we report the structural organization and the promoter activity of the human ILK-1 gene, contained within a 8.8-kb genomic fragment cloned from a human BAC library. The mature protein is encoded by 13 exons. The last coding exon contains the entire 3′ UTR of the ILK-1 gene, which overlaps with the complementary 3′ UTR sequence of the TAF2H gene, a TATA box binding protein-associated factor. A major transcriptional initiation start site was found 138 bp upstream of exon 1 in close proximity to a consensus initiator element (Inr). The ILK gene is transcribed by a TATA-less and CAAT-less promoter with typical features of housekeeping genes. The promoter activity was characterized by a luciferase reporter assay and the minimal sequence conferring promoter activity was 349 bp in size and located immediately upstream of exon 1.
AB - Integrin-linked kinase (ILK)-1 is a 59-kDa serine-threonine protein kinase, which associates with the cytoplasmic domain of β1, β2 and β3 integrins and acts as a receptor proximal kinase regulating integrin-mediated signal transduction. We have recently identified an isoform of ILK (ILK-2), which is expressed, in a TGF-β1-dependent manner, in a highly invasive tumor cell line but not in normal adult tissues. In contrast, ILK-1 is ubiquitously expressed in normal tissues and is up-regulated in various tumors independent of TGF-β1. Here, we report the structural organization and the promoter activity of the human ILK-1 gene, contained within a 8.8-kb genomic fragment cloned from a human BAC library. The mature protein is encoded by 13 exons. The last coding exon contains the entire 3′ UTR of the ILK-1 gene, which overlaps with the complementary 3′ UTR sequence of the TAF2H gene, a TATA box binding protein-associated factor. A major transcriptional initiation start site was found 138 bp upstream of exon 1 in close proximity to a consensus initiator element (Inr). The ILK gene is transcribed by a TATA-less and CAAT-less promoter with typical features of housekeeping genes. The promoter activity was characterized by a luciferase reporter assay and the minimal sequence conferring promoter activity was 349 bp in size and located immediately upstream of exon 1.
KW - Genomic sequence
KW - Integrin
KW - Integrin-linked kinase
KW - Promoter activity
UR - http://www.scopus.com/inward/record.url?scp=0037012729&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(02)00247-6
DO - 10.1016/S0167-4781(02)00247-6
M3 - Article
C2 - 12020826
AN - SCOPUS:0037012729
SN - 0167-4781
VL - 1575
SP - 117
EP - 122
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 1-3
ER -