Prevalence of HIV co-receptor polymorphisms in HIV-infected patients and uninfected volunteers in Luxembourg

Purpose: To describe the prevalence of nine HIV-1 co-receptor polymorphisms in Luxembourg (CCR5-Δ32, CCR5-58755-A/G, CCR5m303, CCR5-59029-A/G, CCR2-641, CCR5-59653-C/T, CX₃CR1-V2491, CX₃CR1-T280M, and SDF1-3′A) in 288 HIV-1-infected patients and in 158 uninfected, healthy volunteers. Method: The presence of mutations was detected using PCR-restriction fragment length polymorphism (RFLP)-based methods. Results: We did not find any significant differences in genotype distributions and allele frequencies between infected and uninfected participants in Luxembourg. The distribution of genotypes agreed in all groups with Hardy-Weinberg predicted proportions. CCR5-Δ32 was significantly associated with HIV-1-infected slow progressor patients. Conclusion: Overall, allele frequencies were comparable to frequencies reported in previous studies in Caucasian populations.