TY - JOUR
T1 - Prevalence of high risk hpv dna in esophagus is high in Brazil but not related to esophageal squamous cell carcinoma
AU - Da Costa, Allini Mafra
AU - Fregnani, José Humberto Tavares Guerreiro
AU - Pastrez, Paula Roberta Aguiar
AU - Mariano, Vânia Sammartino
AU - Neto, Cristovam Scapulatempo
AU - Guimarães, Denise Peixoto
AU - De Oliveira, Kelly Menezio Giordina
AU - Neto, Said Abdala Zemi
AU - Nunes, Emily Montosa
AU - Ferreira, Silvaneide
AU - Sichero, Laura
AU - Villa, Luisa Lina
AU - Syrjanen, Kari Juhani
AU - Longatto-Filho, Adhemar
N1 - Funding Information:
Acknowledgements: The authors would like to thank the BCH, Medical Specialties Ambulatory (AME) Clinic of Barretos, HPV Teams at BCH and Molecular Biology Laboratory, Center for Translational Research in Oncology, São Paulo Cancer Institute (ICESP), São Paulo, Brazil. Funding statement: CNPq Universal for providing supplies to the largest study, of which this study is a part of, entitled “The role of human papillomavirus (HPV) as the etiologic agent of esophageal cancer. A cross-sectional study, case-control and longitudinal at Barretos Cancer Hospital”; (Grant number 482666/2012-9 to ALF); INCT HPV [Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) [Grant number 08/57889-1 to LLV]; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant number 573799/2008-3 to LLV)]. Ethical Aspects and Consent to Participate: This study was approved by the Ethics Committee at BCH with register number 134471. Patients were referred to a private room and informed about the purpose of the study, procedures for biological sampling and the necessary information requested on the data collection instrument. Patients who agreed to participate signed the Informed Consent form. Participants, then, answered a questionnaire containing sociodemographic information and explaining the known risk factors for the HPV infection and EC. All interviews were conducted by previously trained members of the HPV Research Group at BCH - Pius XII Foundation. Interviews provided information to characterize the duration and type of exposure. Conflict of interest: Nothing to declare.
Publisher Copyright:
© 2018, Histology and Histopathology. All rights reserved.
PY - 2018/4
Y1 - 2018/4
N2 - Background. The first publication that associated Human Papillomavirus (HPV) infection and esophageal cancer was published in 1982. However, data are still contradictory and require further investigation. The aim of this study was to identify high risk HPV DNA in esophageal tissue of patients with and without esophageal squamous cell carcinoma (ESCC) and correlate HPV presence with classical risk factors. Methods. Invited patients signed the informed consent form, and interviews were conducted in order to obtain information about sociodemographic and lifestyle behavior. During endoscopy, esophageal biopsies were collected from case and controls. Multiplex polymerase chain reaction genotyping was conducted on endoscopic biopsies to identify HPV types and HPV-16 was further evaluated by specific PCR real time. Results. Among 87 cases, 12 (13.8%) had tumors harboring high risk HPV DNA and among 87 controls, 12 (13.8%) had high risk HPV DNA (OR:1.025 [CI:0.405:2.592]). Variables regarding consumption of alcohol and use of tobacco continued to characterize risk factors even after adjustments by presence or absence of high risk HPV. Conclusion. HPV was demonstrated to be frequently and similarly associated to normal and malignant esophageal tissues, but not as an independent risk factor to esophageal cancer. Impact. To contribute to the Brazilian population data on this subject, which is still contradictory.
AB - Background. The first publication that associated Human Papillomavirus (HPV) infection and esophageal cancer was published in 1982. However, data are still contradictory and require further investigation. The aim of this study was to identify high risk HPV DNA in esophageal tissue of patients with and without esophageal squamous cell carcinoma (ESCC) and correlate HPV presence with classical risk factors. Methods. Invited patients signed the informed consent form, and interviews were conducted in order to obtain information about sociodemographic and lifestyle behavior. During endoscopy, esophageal biopsies were collected from case and controls. Multiplex polymerase chain reaction genotyping was conducted on endoscopic biopsies to identify HPV types and HPV-16 was further evaluated by specific PCR real time. Results. Among 87 cases, 12 (13.8%) had tumors harboring high risk HPV DNA and among 87 controls, 12 (13.8%) had high risk HPV DNA (OR:1.025 [CI:0.405:2.592]). Variables regarding consumption of alcohol and use of tobacco continued to characterize risk factors even after adjustments by presence or absence of high risk HPV. Conclusion. HPV was demonstrated to be frequently and similarly associated to normal and malignant esophageal tissues, but not as an independent risk factor to esophageal cancer. Impact. To contribute to the Brazilian population data on this subject, which is still contradictory.
KW - Cancer Epidemiology
KW - Esophageal cancer
KW - Esophageal neoplasms
KW - Human papillomavirus
KW - Risk factors
UR - http://www.scopus.com/inward/record.url?scp=85041647519&partnerID=8YFLogxK
U2 - 10.14670/HH-11-929
DO - 10.14670/HH-11-929
M3 - Article
C2 - 28875493
AN - SCOPUS:85041647519
SN - 0213-3911
VL - 33
SP - 357
EP - 363
JO - Histology and Histopathology
JF - Histology and Histopathology
IS - 4
ER -