Phages and HIV-1: From display to interplay

Sylvie Delhalle*, Jean Claude Schmit, Andy Chevigné

*Corresponding author for this work

    Research output: Contribution to journalArticleResearchpeer-review

    18 Citations (Scopus)

    Abstract

    The complex hide-and-seek game between HIV-1 and the host immune system has impaired the development of an efficient vaccine. In addition, the high variability of the virus impedes the long-term control of viral replication by small antiviral drugs. For more than 20 years, phage display technology has been intensively used in the field of HIV-1 to explore the epitope landscape recognized by monoclonal and polyclonal HIV-1-specific antibodies, thereby providing precious data about immunodominant and neutralizing epitopes. In parallel, biopanning experiments with various combinatorial or antibody fragment libraries were conducted on viral targets as well as host receptors to identify HIV-1 inhibitors. Besides these applications, phage display technology has been applied to characterize the enzymatic specificity of the HIV-1 protease. Phage particles also represent valuable alternative carriers displaying various HIV-1 antigens to the immune system and eliciting antiviral responses. This review presents and summarizes the different studies conducted with regard to the nature of phage libraries, target display mode and biopanning procedures.

    Original languageEnglish
    Pages (from-to)4727-4794
    Number of pages68
    JournalInternational Journal of Molecular Sciences
    Volume13
    Issue number4
    DOIs
    Publication statusPublished - Apr 2012

    Keywords

    • CCR5
    • CXCR4
    • Epitope mapping
    • Gp120
    • Gp41
    • HIV-1
    • HIV-1 inhibitor
    • HIV-1 vaccine
    • Mimotopes
    • Phage display

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