Pan-claudin family interactome analysis reveals shared and specific interactions

Lorena Suarez-Artiles, Tilman Breiderhoff, Rossana Girardello, Hannes Gonschior, Sophie Rodius, Antoine Lesur, Ulf Reimer, Evelyn Ramberger, Daniel Perez-Hernandez, Dominik Müller*, Philipp Mertins*, Gunnar Dittmar*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Claudins are a family of transmembrane proteins expressed in epithelial tissues and are the major components of tight junctions (TJs), which define barrier properties in epithelia and maintain cell polarity. How claudins regulate the formation of TJs and which functions they exert outside of them is not entirely understood. Although the long and unstructured C-terminal tail is essential for regulation, it is unclear how it is involved in these functions beyond interacting with TJ-associated proteins such as TJ protein ZO-1 (TJP1). Here, we present an interactome study of the pan-claudin family in Madin-Darby canine kidney (MDCK)-C7 cells by combining two complementary mass spectrometry-based pull-down techniques creating an interaction landscape of the entire claudin family. The interaction partners of the claudins' C termini reveal their possible implications in localized biological processes in epithelial cells and their regulation by post-translational modifications (PTMs).

Original languageEnglish
Pages (from-to)111588
JournalCell Reports
Volume41
Issue number6
DOIs
Publication statusPublished - 8 Nov 2022

Keywords

  • Dogs
  • Animals
  • Claudins/metabolism
  • Cell Line
  • Tight Junctions/metabolism
  • Madin Darby Canine Kidney Cells
  • Cell Polarity

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