TY - JOUR
T1 - Nuclear Retention of Unspliced mRNAs in Yeast Is Mediated by Perinuclear Mlp1
AU - Galy, Vincent
AU - Gadal, Olivier
AU - Fromont-Racine, Micheline
AU - Romano, Alper
AU - Jacquier, Alain
AU - Nehrbass, Ulf
N1 - Funding Information:
We thank Frank Feuerbach-Fournier for useful suggestions in the course of this work, Drs. Annette Boese, Sophie Bachellier, and John Aitchison for critical reading of the manuscript. O.G. has been supported by a Bourse Roux fellowship from Institut Pasteur, V.G. by a fellowship from ARC, and A.R. by a Cantarini fellowship from Institut Pasteur.
PY - 2004/1/9
Y1 - 2004/1/9
N2 - The molecular mechanism underlying the retention of intron-containing mRNAs in the nucleus is not understood. Here, we show that retention of intron-containing mRNAs in yeast is mediated by perinuclearly located Mlp1. Deletion of MLP1 impairs retention while having no effect on mRNA splicing. The Mlp1-dependent leakage of intron-containing RNAs is increased in presence of ts-prp18Δ, a splicing mutant. When overall pre-mRNA levels are increased by deletion of RRP6, a nuclear exosome component, MLP1 deletion augments leakage of only the intron-containing portion of mRNAs. Our data suggest, moreover, that Mlp1-dependent retention is mediated via the 5′ splice site. Intriguingly, we found Mlp-proteins to be present only on sections of the NE adjacent to chromatin. We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs.
AB - The molecular mechanism underlying the retention of intron-containing mRNAs in the nucleus is not understood. Here, we show that retention of intron-containing mRNAs in yeast is mediated by perinuclearly located Mlp1. Deletion of MLP1 impairs retention while having no effect on mRNA splicing. The Mlp1-dependent leakage of intron-containing RNAs is increased in presence of ts-prp18Δ, a splicing mutant. When overall pre-mRNA levels are increased by deletion of RRP6, a nuclear exosome component, MLP1 deletion augments leakage of only the intron-containing portion of mRNAs. Our data suggest, moreover, that Mlp1-dependent retention is mediated via the 5′ splice site. Intriguingly, we found Mlp-proteins to be present only on sections of the NE adjacent to chromatin. We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs.
UR - http://www.scopus.com/inward/record.url?scp=0742304306&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(03)01026-2
DO - 10.1016/S0092-8674(03)01026-2
M3 - Article
C2 - 14718167
AN - SCOPUS:0742304306
SN - 0092-8674
VL - 116
SP - 63
EP - 73
JO - Cell
JF - Cell
IS - 1
ER -