Nuclear Retention of Unspliced mRNAs in Yeast Is Mediated by Perinuclear Mlp1

Vincent Galy, Olivier Gadal, Micheline Fromont-Racine, Alper Romano, Alain Jacquier*, Ulf Nehrbass

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

266 Citations (Scopus)

Abstract

The molecular mechanism underlying the retention of intron-containing mRNAs in the nucleus is not understood. Here, we show that retention of intron-containing mRNAs in yeast is mediated by perinuclearly located Mlp1. Deletion of MLP1 impairs retention while having no effect on mRNA splicing. The Mlp1-dependent leakage of intron-containing RNAs is increased in presence of ts-prp18Δ, a splicing mutant. When overall pre-mRNA levels are increased by deletion of RRP6, a nuclear exosome component, MLP1 deletion augments leakage of only the intron-containing portion of mRNAs. Our data suggest, moreover, that Mlp1-dependent retention is mediated via the 5′ splice site. Intriguingly, we found Mlp-proteins to be present only on sections of the NE adjacent to chromatin. We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs.

Original languageEnglish
Pages (from-to)63-73
Number of pages11
JournalCell
Volume116
Issue number1
DOIs
Publication statusPublished - 9 Jan 2004
Externally publishedYes

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