TY - JOUR
T1 - Nuclear receptors in human immune cells
T2 - Expression and correlations
AU - Schote, Andrea B.
AU - Turner, Jonathan D.
AU - Schiltz, Jang
AU - Muller, Claude P.
N1 - Funding Information:
We would like to thank Dirk Hellhammer for his support and initiatives within the Graduate School of Psychobiology of the University of Trier (Germany). We are grateful to Dr. Marc Keipes and the surgical team at the Clinic St. Therese, Luxembourg for the biopsy samples. This work was supported by the Luxemburg Ministry of Education (BFR 04/058) to ABS and postdoctoral fellowship to JDT from the Ministry of Health. The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work.
PY - 2007/2
Y1 - 2007/2
N2 - Nuclear receptors (NR) are key modulators of gene transcription. Their activity is ligand induced and modulates a large variety of tissue-specific cellular functions. However, for many NR little is known about their role in cells of the immune system. In this study, expression patterns and distribution of 24 NR were investigated in human peripheral blood mononuclear cells. We provide the first evidence of the expression of the 12 receptors CAR, CoupTFα, CoupTFβ, FXR, GCNF, HNF4α, PPARβ/δ, PXR, RevErbβ, TR2, TR4 and TLX in highly purified CD4, CD8, CD19, CD14 cells. The expression profile of RevErbα and LXRα previously observed in B cell and macrophages, respectively, has been extended to CD4, CD8 and CD14 cells. Except for RARβ, which was absence in any of the cells tested, our results suggest an almost ubiquitous expression of the NR in the different cell lineages of the immune system. The expression of CAR, CoupTFα, FXR was also confirmed at a protein level and despite conspicuous mRNA levels of HNF4α, only low levels of this receptor were detectable in the nuclear fraction of PBMCs. Expression of the latter receptors was mostly only a fraction (4-20%) of their expression in the thyroid gland, the adrenal gland, the lung or subcutaneous adipose tissue. The Spearman rank order correlation test was performed to examine the correlation in expression between individual nuclear receptor pairs in the four cell types for several donors. Distinct correlation patterns were observed between receptor pairs in the individual cell types. In CD4 T cells four NR, GCNF, PPARγ, PPARα7 and RevErbβ are perfectly correlated with each other (P ≥ 0.0167). In the other cell types correlations between NR pairs were more diverse, but also statistically highly significant. Interestingly, the relative expression level of a number of receptor pairs ranked identical or similar in at least three (CoupTFα and PPARβ/δ, CoupTFβ and HNF4α as well as RORβ and PXR) or four cell types (CoupTFα and CoupTFβ, PPARγ and RevErbβ). Despite the variability of NR expression in immune cells, these results suggest that some of the NR may be co-regulated in human immune cells.
AB - Nuclear receptors (NR) are key modulators of gene transcription. Their activity is ligand induced and modulates a large variety of tissue-specific cellular functions. However, for many NR little is known about their role in cells of the immune system. In this study, expression patterns and distribution of 24 NR were investigated in human peripheral blood mononuclear cells. We provide the first evidence of the expression of the 12 receptors CAR, CoupTFα, CoupTFβ, FXR, GCNF, HNF4α, PPARβ/δ, PXR, RevErbβ, TR2, TR4 and TLX in highly purified CD4, CD8, CD19, CD14 cells. The expression profile of RevErbα and LXRα previously observed in B cell and macrophages, respectively, has been extended to CD4, CD8 and CD14 cells. Except for RARβ, which was absence in any of the cells tested, our results suggest an almost ubiquitous expression of the NR in the different cell lineages of the immune system. The expression of CAR, CoupTFα, FXR was also confirmed at a protein level and despite conspicuous mRNA levels of HNF4α, only low levels of this receptor were detectable in the nuclear fraction of PBMCs. Expression of the latter receptors was mostly only a fraction (4-20%) of their expression in the thyroid gland, the adrenal gland, the lung or subcutaneous adipose tissue. The Spearman rank order correlation test was performed to examine the correlation in expression between individual nuclear receptor pairs in the four cell types for several donors. Distinct correlation patterns were observed between receptor pairs in the individual cell types. In CD4 T cells four NR, GCNF, PPARγ, PPARα7 and RevErbβ are perfectly correlated with each other (P ≥ 0.0167). In the other cell types correlations between NR pairs were more diverse, but also statistically highly significant. Interestingly, the relative expression level of a number of receptor pairs ranked identical or similar in at least three (CoupTFα and PPARβ/δ, CoupTFβ and HNF4α as well as RORβ and PXR) or four cell types (CoupTFα and CoupTFβ, PPARγ and RevErbβ). Despite the variability of NR expression in immune cells, these results suggest that some of the NR may be co-regulated in human immune cells.
KW - B lymphocytes
KW - Expression
KW - Human nuclear receptors
KW - Monocytes
KW - Peripheral blood lymphocytes
KW - T lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=33748809238&partnerID=8YFLogxK
U2 - 10.1016/j.molimm.2006.04.021
DO - 10.1016/j.molimm.2006.04.021
M3 - Article
C2 - 16837048
AN - SCOPUS:33748809238
SN - 0161-5890
VL - 44
SP - 1436
EP - 1445
JO - Molecular Immunology
JF - Molecular Immunology
IS - 6
ER -