NSP1: A yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain

Ulf Nehrbass*, Hildegard Kern, Ann Mutvei, Heinz Horstmann, Brigitte Marshallsay, Eduard C. Hurt

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

122 Citations (Scopus)

Abstract

NSP1 is located at the nuclear periphery in yeast and is essential for cell growth. Employing immunoelectron microscopy on yeast cells, we show that NSP1 is located at the nuclear pores. The molecular analysis of the NSP1 protein points to a two domain model: a nonessential domain (the first 603 amino acids) composed of repetitive sequences common to other nuclear proteins and an essential, carboxy-terminal domain (residues 604-823) mediating the vital function of NSP1. The NSP1 carboxy-terminal domain, which shows a heptad repeat organization, affected the correct location of two nuclear proteins: site-specific amino acid substitutions within a predicted α-helical structure of this domain caused a temperature-sensitive growth arrest at 37°C and the appearance of NSP1 and NOP1, a nucleolar protein, in the cytosol.

Original languageEnglish
Pages (from-to)979-989
Number of pages11
JournalCell
Volume61
Issue number6
DOIs
Publication statusPublished - 15 Jun 1990
Externally publishedYes

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