TY - JOUR
T1 - Non-small-cell lung carcinoma cells invade human bronchial mucosa in vitro
AU - Fjellbirkeland, Lars
AU - Bjerkvig, Rolf
AU - Laerum, Ole Didrik
N1 - Funding Information:
This project was supported by the Norwegian Cancer Society, grant no. 92105, Michael Irgens Flock's Legacy and the Astri and Edvard Riiscen's Legacy. The authors wish to thank Professor Amund Gulsvik and his staff at the Department of Thoracic Medicine, Haukeland University Hospital, for supplying the biopsies used in this study. We also appreciate the technical assistance from Geir E. Eide.
PY - 1998
Y1 - 1998
N2 - To study invasion of lung cancer in vitro a novel three-dimensional coculture assay consisting of living human tissues has been developed. Multicellular spheroids initiated from a new large-cell lung carcinoma cell line (GaL23), found to be invasive in immunodeficient mice, were confronted with precultured bronchial fragments derived from mucosal biopsies obtained during routine fiberoptic bronchoscopy. The bronchial fragments consist of a stromal core with scattered fibroblasts covered by a continuous surface epithelium resting on a basal lamina. During the first 2 wk of confrontation, a gradual retraction of the bronchial epithelium with subsequent adhesion of the tumor cells to the underlying basal lamina occurred. The following week, a limited invasion of tumor cells into the bronchial stroma was seen. To facilitate the entrance of tumor cells through the mucosal surface, the surface epithelium was removed prior to coculture by ethylenediaminetetraacetic acid (EDTA) buffer treatment. Upon confrontation, GaL23 cells then rapidly attached to and migrated on the exposed basal lamina and an increasing number of tumor cells was seen in the stroma during the first week of culture. This model offers opportunities for studying mechanisms of lung cancer adhesion, migration, and invasion using human bronchial mucosa as the natural target tissue.
AB - To study invasion of lung cancer in vitro a novel three-dimensional coculture assay consisting of living human tissues has been developed. Multicellular spheroids initiated from a new large-cell lung carcinoma cell line (GaL23), found to be invasive in immunodeficient mice, were confronted with precultured bronchial fragments derived from mucosal biopsies obtained during routine fiberoptic bronchoscopy. The bronchial fragments consist of a stromal core with scattered fibroblasts covered by a continuous surface epithelium resting on a basal lamina. During the first 2 wk of confrontation, a gradual retraction of the bronchial epithelium with subsequent adhesion of the tumor cells to the underlying basal lamina occurred. The following week, a limited invasion of tumor cells into the bronchial stroma was seen. To facilitate the entrance of tumor cells through the mucosal surface, the surface epithelium was removed prior to coculture by ethylenediaminetetraacetic acid (EDTA) buffer treatment. Upon confrontation, GaL23 cells then rapidly attached to and migrated on the exposed basal lamina and an increasing number of tumor cells was seen in the stroma during the first week of culture. This model offers opportunities for studying mechanisms of lung cancer adhesion, migration, and invasion using human bronchial mucosa as the natural target tissue.
KW - Bronchial carcinoma
KW - Cell line
KW - Invasion assay
KW - Organ culture
KW - Spheroid
UR - http://www.scopus.com/inward/record.url?scp=0032441971&partnerID=8YFLogxK
U2 - 10.1007/s11626-998-0010-4
DO - 10.1007/s11626-998-0010-4
M3 - Article
C2 - 9590507
AN - SCOPUS:0032441971
SN - 1071-2690
VL - 34
SP - 333
EP - 340
JO - In Vitro Cellular and Developmental Biology - Animal
JF - In Vitro Cellular and Developmental Biology - Animal
IS - 4
ER -