Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions

Josh P Peters, Arne Brahms, Vivian Janicaud, Maria Anikeeva, Eva Peschke, Frowin Ellermann, Arianna Ferrari, Dana Hellmold, Janka Held-Feindt, Na-Mi Kim, Johannes Meiser, Konrad Aden, Rainer Herges, Jan-Bernd Hövener*, Andrey N Pravdivtsev*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Dissolution dynamic nuclear polarization (dDNP) increases the sensitivity of magnetic resonance imaging by more than 10,000 times, enabling in vivo metabolic imaging to be performed noninvasively in real time. Here, we are developing a group of dDNP polarized tracers based on nicotinamide (NAM). We synthesized 1-15N-NAM and 1-15N nicotinic acid and hyperpolarized them with dDNP, reaching (13.0 ± 1.9)% 15N polarization. We found that the lifetime of hyperpolarized 1-15N-NAM is strongly field- and pH-dependent, with T1 being as long as 41 s at a pH of 12 and 1 T while as short as a few seconds at neutral pH and fields below 1 T. The remarkably short 1-15N lifetime at low magnetic fields and neutral pH drove us to establish a unique pH neutralization procedure. Using 15N dDNP and an inexpensive rodent imaging probe designed in-house, we acquired a 15N MRI of 1-15N-NAM (previously hyperpolarized for more than an hour) in less than 1 s.

Original languageEnglish
Article numbereadd3643
Pages (from-to)eadd3643
JournalScience advances
Volume9
Issue number34
Early online date23 Aug 2023
DOIs
Publication statusPublished - 25 Aug 2023

Keywords

  • Niacinamide/pharmacology
  • Nitrogen Isotopes
  • 2-Naphthylamine

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