Nicotine induces chromatin changes and c-Jun up-regulation in HL-60 leukemia cells

Emilie Landais; Victoria El-Khoury; Alain Prevost; Jean Dufer; Françoise Liautaud-Roger

doi:10.3892/or.14.6.1553

Nicotine induces chromatin changes and c-Jun up-regulation in HL-60 leukemia cells

Emilie Landais, Victoria El-Khoury, Alain Prevost, Jean Dufer, Françoise Liautaud-Roger^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

Although nicotine has been implicated as a potential factor in the pathogenesis of human cancer, its mechanisms of action regarding cancer development remain largely unknown. HL-60 cells were used to investigate the effects of a short-term treatment with nicotine at concentrations found in the blood of smokers. The findings show that nicotine induces chromatin decondensation, histone H3 acetylation and up-regulation of the c-Jun transcription factor mRNA. This increase is inhibited by mecamylamine, a nicotinic receptor antagonist, suggesting that nicotine alters cellular function directly via nicotinic acetylcholine receptors and may then play a role in cell physiology and tumor promotion.

Original language	English
Pages (from-to)	1553-1558
Number of pages	6
Journal	Oncology Reports
Volume	14
Issue number	6
DOIs	https://doi.org/10.3892/or.14.6.1553
Publication status	Published - Dec 2005
Externally published	Yes

Keywords

Chromatin
HL-60 cells
Histone acetylation
Image analysis
Nicotine
Transcription factors

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10.3892/or.14.6.1553

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title = "Nicotine induces chromatin changes and c-Jun up-regulation in HL-60 leukemia cells",

abstract = "Although nicotine has been implicated as a potential factor in the pathogenesis of human cancer, its mechanisms of action regarding cancer development remain largely unknown. HL-60 cells were used to investigate the effects of a short-term treatment with nicotine at concentrations found in the blood of smokers. The findings show that nicotine induces chromatin decondensation, histone H3 acetylation and up-regulation of the c-Jun transcription factor mRNA. This increase is inhibited by mecamylamine, a nicotinic receptor antagonist, suggesting that nicotine alters cellular function directly via nicotinic acetylcholine receptors and may then play a role in cell physiology and tumor promotion.",

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T1 - Nicotine induces chromatin changes and c-Jun up-regulation in HL-60 leukemia cells

AU - Landais, Emilie

AU - El-Khoury, Victoria

AU - Prevost, Alain

AU - Dufer, Jean

AU - Liautaud-Roger, Françoise

PY - 2005/12

Y1 - 2005/12

N2 - Although nicotine has been implicated as a potential factor in the pathogenesis of human cancer, its mechanisms of action regarding cancer development remain largely unknown. HL-60 cells were used to investigate the effects of a short-term treatment with nicotine at concentrations found in the blood of smokers. The findings show that nicotine induces chromatin decondensation, histone H3 acetylation and up-regulation of the c-Jun transcription factor mRNA. This increase is inhibited by mecamylamine, a nicotinic receptor antagonist, suggesting that nicotine alters cellular function directly via nicotinic acetylcholine receptors and may then play a role in cell physiology and tumor promotion.

AB - Although nicotine has been implicated as a potential factor in the pathogenesis of human cancer, its mechanisms of action regarding cancer development remain largely unknown. HL-60 cells were used to investigate the effects of a short-term treatment with nicotine at concentrations found in the blood of smokers. The findings show that nicotine induces chromatin decondensation, histone H3 acetylation and up-regulation of the c-Jun transcription factor mRNA. This increase is inhibited by mecamylamine, a nicotinic receptor antagonist, suggesting that nicotine alters cellular function directly via nicotinic acetylcholine receptors and may then play a role in cell physiology and tumor promotion.

KW - Chromatin

KW - HL-60 cells

KW - Histone acetylation

KW - Image analysis

KW - Nicotine

KW - Transcription factors

UR - https://www.scopus.com/pages/publications/33644682643

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DO - 10.3892/or.14.6.1553

M3 - Article

C2 - 16273255

AN - SCOPUS:33644682643

SN - 1021-335X

VL - 14

SP - 1553

EP - 1558

JO - Oncology Reports

JF - Oncology Reports

IS - 6

ER -

Nicotine induces chromatin changes and c-Jun up-regulation in HL-60 leukemia cells

Abstract

Keywords

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