TY - JOUR
T1 - Navigating the path of reproducibility in microRNA-based biomarker research with ring trials
AU - Sopić, Miron
AU - Devaux, Yvan
AU - De Gonzalo-Calvo, David
AU - AtheroNET COST Action CA21153
N1 - Acknowledgements: This work uses data that have been provided by patients and collected by the NHS as part of their care and support. The data are collated, maintained and quality assured by the National Cancer Registration and Analysis Service. Access to the data was facilitated by the
NHS England’s Data Access Request Service and the Cancer Research UK funded UK Colorectal Cancer Intelligence Hub (C23434/A23706)
Publisher Copyright:
© 2024 Walter de Gruyter GmbH, Berlin/Boston 2024.
PY - 2024/5/16
Y1 - 2024/5/16
N2 - The development of microRNA (miRNA)-based biomarkers has gained significant attention due to their potential diagnostic, prognostic and therapeutic applications. However, the reproducibility of miRNA biomarker research faces unique challenges, primarily due to the influence of pre-analytical and analytical factors. The absence of standardized procedures contributes to inconsistencies across studies, alongside challenges in reference gene selection, data analysis methods and miRNA profiling platforms. Inter-laboratory comparison trials, or ring trials, offer a strategic approach to address technical and biological variability in miRNA biomarker studies. These trials promote standardization, identify sources of variability and strengthen the correlation between miRNAs and clinical outcomes. Despite their underutilization in miRNA biomarker research, ring trials represent a valuable tool for enhancing reproducibility and expediting the translation of miRNA-based biomarkers into clinical applications.
AB - The development of microRNA (miRNA)-based biomarkers has gained significant attention due to their potential diagnostic, prognostic and therapeutic applications. However, the reproducibility of miRNA biomarker research faces unique challenges, primarily due to the influence of pre-analytical and analytical factors. The absence of standardized procedures contributes to inconsistencies across studies, alongside challenges in reference gene selection, data analysis methods and miRNA profiling platforms. Inter-laboratory comparison trials, or ring trials, offer a strategic approach to address technical and biological variability in miRNA biomarker studies. These trials promote standardization, identify sources of variability and strengthen the correlation between miRNAs and clinical outcomes. Despite their underutilization in miRNA biomarker research, ring trials represent a valuable tool for enhancing reproducibility and expediting the translation of miRNA-based biomarkers into clinical applications.
KW - biomarker
KW - microRNA
KW - ring trial
UR - http://www.scopus.com/inward/record.url?scp=85193279826&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/38747410
U2 - 10.1515/cclm-2024-0531
DO - 10.1515/cclm-2024-0531
M3 - Article
C2 - 38747410
AN - SCOPUS:85193279826
SN - 1434-6621
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
ER -