Naturally occurring humoral cytotoxicity against neuroblastoma (NB) cells in healthy persons and NB patients

Rudolf Erttmann; Caroline Schmitt; Markus W. Ollert; Kerstin David; Reinhard Bredehorst; Carl Wilhelm Vogel

doi:10.3109/08880019609030870

Naturally occurring humoral cytotoxicity against neuroblastoma (NB) cells in healthy persons and NB patients

Rudolf Erttmann^*, Caroline Schmitt, Markus W. Ollert, Kerstin David, Reinhard Bredehorst, Carl Wilhelm Vogel

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

A novel cytotoxic mechanism against neuroblastoma (NB) cells based on natural immunoglobulin M serum antibodies is described. The occurrence of these antibodies, which are able to induce complement-mediated cytolysis of NB cells, has been investigated in several age groups of healthy persons and in patients with NB. The cytotoxicity of serum samples has been measured in terms of propidium iodide DNA incorporation using a FACScan. The prevalence of anti-NB IgM antibodies shows a strong age dependence characterized by a nearly complete lack in the first year of life and a steep rise up to 82% beginning with the second year of life. In the sera of 11 NB-bearing patients no cytotoxicity has been found, despite the fact that 8 of these patients were older than 1 year. These findings lead to the hypothesis that natural IgM antibodies could play a role as an immunological control mechanism against NB.

Original language	English
Pages (from-to)	545-548
Number of pages	4
Journal	Pediatric Hematology and Oncology
Volume	13
Issue number	6
DOIs	https://doi.org/10.3109/08880019609030870
Publication status	Published - 1996
Externally published	Yes

Keywords

cytotoxic immunoglobulins
natural immunity
neuroblastoma

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title = "Naturally occurring humoral cytotoxicity against neuroblastoma (NB) cells in healthy persons and NB patients",

abstract = "A novel cytotoxic mechanism against neuroblastoma (NB) cells based on natural immunoglobulin M serum antibodies is described. The occurrence of these antibodies, which are able to induce complement-mediated cytolysis of NB cells, has been investigated in several age groups of healthy persons and in patients with NB. The cytotoxicity of serum samples has been measured in terms of propidium iodide DNA incorporation using a FACScan. The prevalence of anti-NB IgM antibodies shows a strong age dependence characterized by a nearly complete lack in the first year of life and a steep rise up to 82% beginning with the second year of life. In the sera of 11 NB-bearing patients no cytotoxicity has been found, despite the fact that 8 of these patients were older than 1 year. These findings lead to the hypothesis that natural IgM antibodies could play a role as an immunological control mechanism against NB.",

keywords = "cytotoxic immunoglobulins, natural immunity, neuroblastoma",

author = "Rudolf Erttmann and Caroline Schmitt and Ollert, {Markus W.} and Kerstin David and Reinhard Bredehorst and Vogel, {Carl Wilhelm}",

note = "Funding Information: Received 5 February 1996; accepted 1 April 1996. The study was supported by “Forderverein Kinderkrebszentruni Hamburg.” Address correspondence to Professor Dr. med. Rudolf Erttmann, Department of Pediatric Hematology and Oncology, University Children{\textquoteright}s Hospital. MartinistraRe 52, D-20246 Hamburg, Germany.",

year = "1996",

doi = "10.3109/08880019609030870",

language = "English",

volume = "13",

pages = "545--548",

journal = "Pediatric Hematology and Oncology",

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T1 - Naturally occurring humoral cytotoxicity against neuroblastoma (NB) cells in healthy persons and NB patients

AU - Erttmann, Rudolf

AU - Schmitt, Caroline

AU - Ollert, Markus W.

AU - David, Kerstin

AU - Bredehorst, Reinhard

AU - Vogel, Carl Wilhelm

N1 - Funding Information: Received 5 February 1996; accepted 1 April 1996. The study was supported by “Forderverein Kinderkrebszentruni Hamburg.” Address correspondence to Professor Dr. med. Rudolf Erttmann, Department of Pediatric Hematology and Oncology, University Children’s Hospital. MartinistraRe 52, D-20246 Hamburg, Germany.

PY - 1996

Y1 - 1996

N2 - A novel cytotoxic mechanism against neuroblastoma (NB) cells based on natural immunoglobulin M serum antibodies is described. The occurrence of these antibodies, which are able to induce complement-mediated cytolysis of NB cells, has been investigated in several age groups of healthy persons and in patients with NB. The cytotoxicity of serum samples has been measured in terms of propidium iodide DNA incorporation using a FACScan. The prevalence of anti-NB IgM antibodies shows a strong age dependence characterized by a nearly complete lack in the first year of life and a steep rise up to 82% beginning with the second year of life. In the sera of 11 NB-bearing patients no cytotoxicity has been found, despite the fact that 8 of these patients were older than 1 year. These findings lead to the hypothesis that natural IgM antibodies could play a role as an immunological control mechanism against NB.

AB - A novel cytotoxic mechanism against neuroblastoma (NB) cells based on natural immunoglobulin M serum antibodies is described. The occurrence of these antibodies, which are able to induce complement-mediated cytolysis of NB cells, has been investigated in several age groups of healthy persons and in patients with NB. The cytotoxicity of serum samples has been measured in terms of propidium iodide DNA incorporation using a FACScan. The prevalence of anti-NB IgM antibodies shows a strong age dependence characterized by a nearly complete lack in the first year of life and a steep rise up to 82% beginning with the second year of life. In the sera of 11 NB-bearing patients no cytotoxicity has been found, despite the fact that 8 of these patients were older than 1 year. These findings lead to the hypothesis that natural IgM antibodies could play a role as an immunological control mechanism against NB.

KW - cytotoxic immunoglobulins

KW - natural immunity

KW - neuroblastoma

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SN - 0888-0018

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JO - Pediatric Hematology and Oncology

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IS - 6

ER -

Naturally occurring humoral cytotoxicity against neuroblastoma (NB) cells in healthy persons and NB patients

Abstract

Keywords

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