TY - JOUR
T1 - Multiomics Profiling of Toxins in the Venom of the Amazonian Spider Acanthoscurria juruenicola
AU - Nishiduka, Erika S.
AU - Abreu, Thiago F.
AU - Abukawa, Fernanda Midori
AU - Oliveira, Ursula C.
AU - Tardivo, Caio E.O.
AU - Nascimento, Soraia M.
AU - Meissner, Gabriel O.
AU - Chaim, Olga M.
AU - Juliano, Maria A.
AU - Kitano, Eduardo S.
AU - Zelanis, André
AU - Serrano, Solange M.T.
AU - Da Silva, Pedro I.
AU - Junqueira-De-Azevedo, Inácio L.
AU - Nishiyama-Jr, Milton Y.
AU - Tashima, Alexandre K.
N1 - Publisher Copyright:
© 2022 Authors. All rights reserved.
PY - 2022/11/4
Y1 - 2022/11/4
N2 - Acanthoscurria juruenicola is an Amazonian spider described for the first time almost a century ago. However, little is known about their venom composition. Here, we present a multiomics characterization of A. juruenicola venom by a combination of transcriptomics, proteomics, and peptidomics approaches. Transcriptomics of female venom glands resulted in 93,979 unique assembled mRNA transcript encoding proteins. A total of 92 proteins were identified in the venom by mass spectrometry, including 14 mature cysteine-rich peptides (CRPs). Quantitative analysis showed that CRPs, cysteine-rich secretory proteins, metalloproteases, carbonic anhydrases, and hyaluronidase comprise >90% of the venom proteome. Relative quantification of venom toxins was performed by DIA and DDA, revealing converging profiles of female and male specimens by both methods. Biochemical assays confirmed the presence of active hyaluronidases, phospholipases, and proteases in the venom. Moreover, the venom promoted in vivo paralytic activities in crickets, consistent with the high concentration of CRPs. Overall, we report a comprehensive analysis of the arsenal of toxins of A. juruenicola and highlight their potential biotechnological and pharmacological applications. Mass spectrometry data were deposited to the ProteomeXchange Consortium via the PRIDE repository with the dataset identifier PXD013149 and via the MassIVE repository with the dataset identifier MSV000087777.
AB - Acanthoscurria juruenicola is an Amazonian spider described for the first time almost a century ago. However, little is known about their venom composition. Here, we present a multiomics characterization of A. juruenicola venom by a combination of transcriptomics, proteomics, and peptidomics approaches. Transcriptomics of female venom glands resulted in 93,979 unique assembled mRNA transcript encoding proteins. A total of 92 proteins were identified in the venom by mass spectrometry, including 14 mature cysteine-rich peptides (CRPs). Quantitative analysis showed that CRPs, cysteine-rich secretory proteins, metalloproteases, carbonic anhydrases, and hyaluronidase comprise >90% of the venom proteome. Relative quantification of venom toxins was performed by DIA and DDA, revealing converging profiles of female and male specimens by both methods. Biochemical assays confirmed the presence of active hyaluronidases, phospholipases, and proteases in the venom. Moreover, the venom promoted in vivo paralytic activities in crickets, consistent with the high concentration of CRPs. Overall, we report a comprehensive analysis of the arsenal of toxins of A. juruenicola and highlight their potential biotechnological and pharmacological applications. Mass spectrometry data were deposited to the ProteomeXchange Consortium via the PRIDE repository with the dataset identifier PXD013149 and via the MassIVE repository with the dataset identifier MSV000087777.
KW - Acanthoscurria juruenicola
KW - cysteine-rich peptides
KW - peptidomics
KW - quantitative proteomics
KW - spider
KW - transcriptomics
KW - venom
UR - http://www.scopus.com/inward/record.url?scp=85140607627&partnerID=8YFLogxK
U2 - 10.1021/acs.jproteome.2c00593
DO - 10.1021/acs.jproteome.2c00593
M3 - Article
C2 - 36260604
AN - SCOPUS:85140607627
SN - 1535-3893
VL - 21
SP - 2783
EP - 2797
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 11
ER -