Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro

Michelle Hesler; Leonie Aengenheister; Bernhard Ellinger; Roland Drexel; Susanne Straskraba; Carsten Jost; Sylvia Wagner; Florian Meier; H. von Briesen; Claudia Büchel; P. Wick; Tina Buerki-Thurnherr; Yvonne Kohl

doi:10.1016/j.tiv.2019.104610

Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro

Michelle Hesler, Leonie Aengenheister, Bernhard Ellinger, Roland Drexel, Susanne Straskraba, Carsten Jost, Sylvia Wagner, Florian Meier, H. von Briesen, Claudia Büchel, P. Wick, Tina Buerki-Thurnherr, Yvonne Kohl^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

204 Citations (Scopus)

Abstract

Nanoplastics (NP) and microplastics (MP) accumulate in our environment as a consequence of the massive consumption of plastics. Huge knowledge-gaps exist regarding uptake and fate of plastic particles in micro- and nano-dimensions in humans as well as on their impact on human health. This study investigated the transport and effects of 50 nm and 0.5 μm COOH-modified polystyrene (PS) particles, as representatives for NP and MP, in different biological models in vitro. Acute toxicity and potential translocation of the particles were studied at the human intestinal and placental barrier using advanced in vitro co-culture models. Furthermore, embryotoxicity and genotoxicity were investigated as highly sensitive endpoints. Polystyrene was not acutely toxic in both sizes (nano- and microparticles). No transport across the intestinal and placental barrier but a cellular uptake and intracellular accumulation of PS nano- and microparticles were determined. The particles were identified as weak embryotoxic and non-genotoxic. In contrast to single-organ studies, this multi-endpoint study is providing a data-set with the exact same type of particles to compare organ-specific outcomes. Our study clearly shows the need to investigate other types of plastics as well as towards long-term or chronic effects of plastic particles in different biological models in vitro.

Original language	English
Article number	104610
Journal	Toxicology in Vitro
Volume	61
DOIs	https://doi.org/10.1016/j.tiv.2019.104610
Publication status	Published - Dec 2019
Externally published	Yes

Keywords

Embryotoxicity
Intestinal barrier
Multi-endpoint toxicity study
Nano- and microplastics
Placental barrier
Polystyrene

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10.1016/j.tiv.2019.104610

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Hesler, M., Aengenheister, L., Ellinger, B., Drexel, R., Straskraba, S., Jost, C., Wagner, S., Meier, F., von Briesen, H., Büchel, C., Wick, P., Buerki-Thurnherr, T., & Kohl, Y. (2019). Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro. Toxicology in Vitro, 61, Article 104610. https://doi.org/10.1016/j.tiv.2019.104610

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title = "Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro",

abstract = "Nanoplastics (NP) and microplastics (MP) accumulate in our environment as a consequence of the massive consumption of plastics. Huge knowledge-gaps exist regarding uptake and fate of plastic particles in micro- and nano-dimensions in humans as well as on their impact on human health. This study investigated the transport and effects of 50 nm and 0.5 μm COOH-modified polystyrene (PS) particles, as representatives for NP and MP, in different biological models in vitro. Acute toxicity and potential translocation of the particles were studied at the human intestinal and placental barrier using advanced in vitro co-culture models. Furthermore, embryotoxicity and genotoxicity were investigated as highly sensitive endpoints. Polystyrene was not acutely toxic in both sizes (nano- and microparticles). No transport across the intestinal and placental barrier but a cellular uptake and intracellular accumulation of PS nano- and microparticles were determined. The particles were identified as weak embryotoxic and non-genotoxic. In contrast to single-organ studies, this multi-endpoint study is providing a data-set with the exact same type of particles to compare organ-specific outcomes. Our study clearly shows the need to investigate other types of plastics as well as towards long-term or chronic effects of plastic particles in different biological models in vitro.",

keywords = "Embryotoxicity, Intestinal barrier, Multi-endpoint toxicity study, Nano- and microplastics, Placental barrier, Polystyrene",

author = "Michelle Hesler and Leonie Aengenheister and Bernhard Ellinger and Roland Drexel and Susanne Straskraba and Carsten Jost and Sylvia Wagner and Florian Meier and {von Briesen}, H. and Claudia B{\"u}chel and P. Wick and Tina Buerki-Thurnherr and Yvonne Kohl",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

month = dec,

doi = "10.1016/j.tiv.2019.104610",

language = "English",

volume = "61",

journal = "Toxicology in Vitro",

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Hesler, M, Aengenheister, L, Ellinger, B, Drexel, R, Straskraba, S, Jost, C, Wagner, S, Meier, F, von Briesen, H, Büchel, C, Wick, P, Buerki-Thurnherr, T & Kohl, Y 2019, 'Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro', Toxicology in Vitro, vol. 61, 104610. https://doi.org/10.1016/j.tiv.2019.104610

TY - JOUR

T1 - Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro

AU - Hesler, Michelle

AU - Aengenheister, Leonie

AU - Ellinger, Bernhard

AU - Drexel, Roland

AU - Straskraba, Susanne

AU - Jost, Carsten

AU - Wagner, Sylvia

AU - Meier, Florian

AU - von Briesen, H.

AU - Büchel, Claudia

AU - Wick, P.

AU - Buerki-Thurnherr, Tina

AU - Kohl, Yvonne

PY - 2019/12

Y1 - 2019/12

N2 - Nanoplastics (NP) and microplastics (MP) accumulate in our environment as a consequence of the massive consumption of plastics. Huge knowledge-gaps exist regarding uptake and fate of plastic particles in micro- and nano-dimensions in humans as well as on their impact on human health. This study investigated the transport and effects of 50 nm and 0.5 μm COOH-modified polystyrene (PS) particles, as representatives for NP and MP, in different biological models in vitro. Acute toxicity and potential translocation of the particles were studied at the human intestinal and placental barrier using advanced in vitro co-culture models. Furthermore, embryotoxicity and genotoxicity were investigated as highly sensitive endpoints. Polystyrene was not acutely toxic in both sizes (nano- and microparticles). No transport across the intestinal and placental barrier but a cellular uptake and intracellular accumulation of PS nano- and microparticles were determined. The particles were identified as weak embryotoxic and non-genotoxic. In contrast to single-organ studies, this multi-endpoint study is providing a data-set with the exact same type of particles to compare organ-specific outcomes. Our study clearly shows the need to investigate other types of plastics as well as towards long-term or chronic effects of plastic particles in different biological models in vitro.

AB - Nanoplastics (NP) and microplastics (MP) accumulate in our environment as a consequence of the massive consumption of plastics. Huge knowledge-gaps exist regarding uptake and fate of plastic particles in micro- and nano-dimensions in humans as well as on their impact on human health. This study investigated the transport and effects of 50 nm and 0.5 μm COOH-modified polystyrene (PS) particles, as representatives for NP and MP, in different biological models in vitro. Acute toxicity and potential translocation of the particles were studied at the human intestinal and placental barrier using advanced in vitro co-culture models. Furthermore, embryotoxicity and genotoxicity were investigated as highly sensitive endpoints. Polystyrene was not acutely toxic in both sizes (nano- and microparticles). No transport across the intestinal and placental barrier but a cellular uptake and intracellular accumulation of PS nano- and microparticles were determined. The particles were identified as weak embryotoxic and non-genotoxic. In contrast to single-organ studies, this multi-endpoint study is providing a data-set with the exact same type of particles to compare organ-specific outcomes. Our study clearly shows the need to investigate other types of plastics as well as towards long-term or chronic effects of plastic particles in different biological models in vitro.

KW - Embryotoxicity

KW - Intestinal barrier

KW - Multi-endpoint toxicity study

KW - Nano- and microplastics

KW - Placental barrier

KW - Polystyrene

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U2 - 10.1016/j.tiv.2019.104610

DO - 10.1016/j.tiv.2019.104610

M3 - Article

C2 - 31362040

AN - SCOPUS:85070389200

SN - 0887-2333

VL - 61

JO - Toxicology in Vitro

JF - Toxicology in Vitro

M1 - 104610

ER -

Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro

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