Multi-class pesticide analysis in human hair by gas chromatography tandem (triple quadrupole) mass spectrometry with solid phase microextraction and liquid injection

Guillaume Salquèbre; Claude Schummer; Maurice Millet; Olivier Briand; Brice M.R. Appenzeller

doi:10.1016/j.aca.2011.10.029

Multi-class pesticide analysis in human hair by gas chromatography tandem (triple quadrupole) mass spectrometry with solid phase microextraction and liquid injection

Guillaume Salquèbre, Claude Schummer, Maurice Millet, Olivier Briand, Brice M.R. Appenzeller^*

^*Corresponding author for this work

Human Biomonitoring Research Unit

Research output: Contribution to journal › Article › Research › peer-review

57 Citations (Scopus)

Abstract

A method for the simultaneous detection and quantification of 22 pesticides from different chemical classes was developed using solid-phase microextraction (SPME) and gas chromatography tandem (triple quadrupole) mass spectrometry. Pesticides were extracted from 50mg of pulverized hair with acetonitrile. The extract was submitted to two successive steps of direct immersion-SPME at 30°C and 90°C or to a liquid injection without SPME in order to obtain optimized conditions for each of the 22 analytes investigated. Validation parameters were significantly influenced by both the injection mode (SPME vs liquid injection) and the temperature of SPME. Limits of quantification ranged from 0.05pgmg ^-1 for trifluralin to 10pgmg ^-1 for pentachlorophenol. The application of the validated method to the analysis of samples collected from non-occupationally exposed volunteers demonstrated the presence of pesticides in all the samples tested. Altogether, 13 different analytes were detected at concentration above the limit of quantification.

Original language	English
Pages (from-to)	65-74
Number of pages	10
Journal	Analytica Chimica Acta
Volume	710
DOIs	https://doi.org/10.1016/j.aca.2011.10.029
Publication status	Published - 13 Jan 2012

Keywords

Exposure
GC-MS/MS
Hair
Human biomonitoring
Pesticides
SPME

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10.1016/j.aca.2011.10.029

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@article{3ff0007400324ca5926bc4e18dae9119,

title = "Multi-class pesticide analysis in human hair by gas chromatography tandem (triple quadrupole) mass spectrometry with solid phase microextraction and liquid injection",

abstract = "A method for the simultaneous detection and quantification of 22 pesticides from different chemical classes was developed using solid-phase microextraction (SPME) and gas chromatography tandem (triple quadrupole) mass spectrometry. Pesticides were extracted from 50mg of pulverized hair with acetonitrile. The extract was submitted to two successive steps of direct immersion-SPME at 30°C and 90°C or to a liquid injection without SPME in order to obtain optimized conditions for each of the 22 analytes investigated. Validation parameters were significantly influenced by both the injection mode (SPME vs liquid injection) and the temperature of SPME. Limits of quantification ranged from 0.05pgmg -1 for trifluralin to 10pgmg -1 for pentachlorophenol. The application of the validated method to the analysis of samples collected from non-occupationally exposed volunteers demonstrated the presence of pesticides in all the samples tested. Altogether, 13 different analytes were detected at concentration above the limit of quantification.",

keywords = "Exposure, GC-MS/MS, Hair, Human biomonitoring, Pesticides, SPME",

author = "Guillaume Salqu{\`e}bre and Claude Schummer and Maurice Millet and Olivier Briand and Appenzeller, {Brice M.R.}",

note = "Funding Information: This work was supported in part by the French Agency for Food, Environmental and Occupational Health Safety , in the framework of the Pesticide Residue Observatory action.",

year = "2012",

month = jan,

day = "13",

doi = "10.1016/j.aca.2011.10.029",

language = "English",

volume = "710",

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journal = "Analytica Chimica Acta",

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Multi-class pesticide analysis in human hair by gas chromatography tandem (triple quadrupole) mass spectrometry with solid phase microextraction and liquid injection. / Salquèbre, Guillaume; Schummer, Claude; Millet, Maurice et al.
In: Analytica Chimica Acta, Vol. 710, 13.01.2012, p. 65-74.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Multi-class pesticide analysis in human hair by gas chromatography tandem (triple quadrupole) mass spectrometry with solid phase microextraction and liquid injection

AU - Salquèbre, Guillaume

AU - Schummer, Claude

AU - Millet, Maurice

AU - Briand, Olivier

AU - Appenzeller, Brice M.R.

N1 - Funding Information: This work was supported in part by the French Agency for Food, Environmental and Occupational Health Safety , in the framework of the Pesticide Residue Observatory action.

PY - 2012/1/13

Y1 - 2012/1/13

N2 - A method for the simultaneous detection and quantification of 22 pesticides from different chemical classes was developed using solid-phase microextraction (SPME) and gas chromatography tandem (triple quadrupole) mass spectrometry. Pesticides were extracted from 50mg of pulverized hair with acetonitrile. The extract was submitted to two successive steps of direct immersion-SPME at 30°C and 90°C or to a liquid injection without SPME in order to obtain optimized conditions for each of the 22 analytes investigated. Validation parameters were significantly influenced by both the injection mode (SPME vs liquid injection) and the temperature of SPME. Limits of quantification ranged from 0.05pgmg -1 for trifluralin to 10pgmg -1 for pentachlorophenol. The application of the validated method to the analysis of samples collected from non-occupationally exposed volunteers demonstrated the presence of pesticides in all the samples tested. Altogether, 13 different analytes were detected at concentration above the limit of quantification.

AB - A method for the simultaneous detection and quantification of 22 pesticides from different chemical classes was developed using solid-phase microextraction (SPME) and gas chromatography tandem (triple quadrupole) mass spectrometry. Pesticides were extracted from 50mg of pulverized hair with acetonitrile. The extract was submitted to two successive steps of direct immersion-SPME at 30°C and 90°C or to a liquid injection without SPME in order to obtain optimized conditions for each of the 22 analytes investigated. Validation parameters were significantly influenced by both the injection mode (SPME vs liquid injection) and the temperature of SPME. Limits of quantification ranged from 0.05pgmg -1 for trifluralin to 10pgmg -1 for pentachlorophenol. The application of the validated method to the analysis of samples collected from non-occupationally exposed volunteers demonstrated the presence of pesticides in all the samples tested. Altogether, 13 different analytes were detected at concentration above the limit of quantification.

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Multi-class pesticide analysis in human hair by gas chromatography tandem (triple quadrupole) mass spectrometry with solid phase microextraction and liquid injection

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