Mouse phenotyping

Helmut Fuchs; Valérie Gailus-Durner; Thure Adler; Juan Antonio Aguilar-Pimentel; Lore Becker; Julia Calzada-Wack; Patricia Da Silva-Buttkus; Frauke Neff; Alexander Götz; Wolfgang Hans; Sabine M. Hölter; Marion Horsch; Gabi Kastenmüller; Elisabeth Kemter; Christoph Lengger; Holger Maier; Mikolaj Matloka; Gabriele Möller; Beatrix Naton; Cornelia Prehn; Oliver Puk; Ildikó Rácz; Birgit Rathkolb; Werner Römisch-Margl; Jan Rozman; Rui Wang-Sattler; Anja Schrewe; Claudia Stöger; Monica Tost; Jerzy Adamski; Bernhard Aigner; Johannes Beckers; Heidrun Behrendt; Dirk H. Busch; Irene Esposito; Jochen Graw; Thomas Illig; Boris Ivandic; Martin Klingenspor; Thomas Klopstock; Elisabeth Kremmer; Martin Mempel; Susanne Neschen; Markus Ollert; Holger Schulz; Karsten Suhre; Eckhard Wolf; Wolfgang Wurst; Andreas Zimmer; Martin Hrabě de Angelis

doi:10.1016/j.ymeth.2010.08.006

Mouse phenotyping

Helmut Fuchs, Valérie Gailus-Durner, Thure Adler, Juan Antonio Aguilar-Pimentel, Lore Becker, Julia Calzada-Wack, Patricia Da Silva-Buttkus, Frauke Neff, Alexander Götz, Wolfgang Hans, Sabine M. Hölter, Marion Horsch, Gabi Kastenmüller, Elisabeth Kemter, Christoph Lengger, Holger Maier, Mikolaj Matloka, Gabriele Möller, Beatrix Naton, Cornelia PrehnOliver Puk, Ildikó Rácz, Birgit Rathkolb, Werner Römisch-Margl, Jan Rozman, Rui Wang-Sattler, Anja Schrewe, Claudia Stöger, Monica Tost, Jerzy Adamski, Bernhard Aigner, Johannes Beckers, Heidrun Behrendt, Dirk H. Busch, Irene Esposito, Jochen Graw, Thomas Illig, Boris Ivandic, Martin Klingenspor, Thomas Klopstock, Elisabeth Kremmer, Martin Mempel, Susanne Neschen, Markus Ollert, Holger Schulz, Karsten Suhre, Eckhard Wolf, Wolfgang Wurst, Andreas Zimmer, Martin Hrabě de Angelis^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

122 Citations (Scopus)

Abstract

Model organisms like the mouse are important tools to learn more about gene function in man. Within the last 20. years many mutant mouse lines have been generated by different methods such as ENU mutagenesis, constitutive and conditional knock-out approaches, knock-down, introduction of human genes, and knock-in techniques, thus creating models which mimic human conditions. Due to pleiotropic effects, one gene may have different functions in different organ systems or time points during development. Therefore mutant mouse lines have to be phenotyped comprehensively in a highly standardized manner to enable the detection of phenotypes which might otherwise remain hidden. The German Mouse Clinic (GMC) has been established at the Helmholtz Zentrum München as a phenotyping platform with open access to the scientific community (www.mousclinic.de; [1]). The GMC is a member of the EUMODIC consortium which created the European standard workflow EMPReSSslim for the systemic phenotyping of mouse models (http://www.eumodic.org/ [2]).

Original language	English
Pages (from-to)	120-135
Number of pages	16
Journal	Methods
Volume	53
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2010.08.006
Publication status	Published - Feb 2011
Externally published	Yes

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10.1016/j.ymeth.2010.08.006

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Fuchs, H., Gailus-Durner, V., Adler, T., Aguilar-Pimentel, J. A., Becker, L., Calzada-Wack, J., Da Silva-Buttkus, P., Neff, F., Götz, A., Hans, W., Hölter, S. M., Horsch, M., Kastenmüller, G., Kemter, E., Lengger, C., Maier, H., Matloka, M., Möller, G., Naton, B., ... Hrabě de Angelis, M. (2011). Mouse phenotyping. Methods, 53(2), 120-135. https://doi.org/10.1016/j.ymeth.2010.08.006

@article{8795882a6ceb46aa8faecd43002f76b7,

title = "Mouse phenotyping",

abstract = "Model organisms like the mouse are important tools to learn more about gene function in man. Within the last 20. years many mutant mouse lines have been generated by different methods such as ENU mutagenesis, constitutive and conditional knock-out approaches, knock-down, introduction of human genes, and knock-in techniques, thus creating models which mimic human conditions. Due to pleiotropic effects, one gene may have different functions in different organ systems or time points during development. Therefore mutant mouse lines have to be phenotyped comprehensively in a highly standardized manner to enable the detection of phenotypes which might otherwise remain hidden. The German Mouse Clinic (GMC) has been established at the Helmholtz Zentrum M{\"u}nchen as a phenotyping platform with open access to the scientific community (www.mousclinic.de; [1]). The GMC is a member of the EUMODIC consortium which created the European standard workflow EMPReSSslim for the systemic phenotyping of mouse models (http://www.eumodic.org/ [2]).",

author = "Helmut Fuchs and Val{\'e}rie Gailus-Durner and Thure Adler and Aguilar-Pimentel, {Juan Antonio} and Lore Becker and Julia Calzada-Wack and {Da Silva-Buttkus}, Patricia and Frauke Neff and Alexander G{\"o}tz and Wolfgang Hans and H{\"o}lter, {Sabine M.} and Marion Horsch and Gabi Kastenm{\"u}ller and Elisabeth Kemter and Christoph Lengger and Holger Maier and Mikolaj Matloka and Gabriele M{\"o}ller and Beatrix Naton and Cornelia Prehn and Oliver Puk and Ildik{\'o} R{\'a}cz and Birgit Rathkolb and Werner R{\"o}misch-Margl and Jan Rozman and Rui Wang-Sattler and Anja Schrewe and Claudia St{\"o}ger and Monica Tost and Jerzy Adamski and Bernhard Aigner and Johannes Beckers and Heidrun Behrendt and Busch, {Dirk H.} and Irene Esposito and Jochen Graw and Thomas Illig and Boris Ivandic and Martin Klingenspor and Thomas Klopstock and Elisabeth Kremmer and Martin Mempel and Susanne Neschen and Markus Ollert and Holger Schulz and Karsten Suhre and Eckhard Wolf and Wolfgang Wurst and Andreas Zimmer and {Hrab{\v e} de Angelis}, Martin",

note = "Funding Information: We would like to thank Reinhard Seeliger, Miriam Backs, Nicole Boche, Sabrina Bothur, Ralf Fischer, Michaela Grandl, Tamara Halex, Sabine Holthaus, Elfi Holupirek, Constanze K{\"o}nig, Maria Kugler, Albert Langer, Katrin Laube, Astrid Markert, Jacqueline M{\"u}ller, Elenore Samson, Sandra Sch{\"a}dler, Florian Schleicher, Daniela Schmidt, Waldemar Schneider, Ann-Elisabeth Schwarz, Anett Seelig, Bettina Sperling, Waltraud Stettinger, Lucie Thurmann, Susanne Wittich, Anja Wohlbier and Claudia Zeller as well as the GMC animal caretaker team for expert technical help. This work has been funded by the BMBF (NGFNplus Grant, 01GS08156 , 01GS0850 , 01GS0851 , 01GS0852 , 01GS0868 , 01GS0869 , 01GS0853 , and 01GS0854 ) and by an EU grant (EUMODIC, LSHG-2006-037188 , German Mouse Clinic).",

year = "2011",

month = feb,

doi = "10.1016/j.ymeth.2010.08.006",

language = "English",

volume = "53",

pages = "120--135",

journal = "Methods",

issn = "1046-2023",

publisher = "Academic Press Inc.",

number = "2",

}

Fuchs, H, Gailus-Durner, V, Adler, T, Aguilar-Pimentel, JA, Becker, L, Calzada-Wack, J, Da Silva-Buttkus, P, Neff, F, Götz, A, Hans, W, Hölter, SM, Horsch, M, Kastenmüller, G, Kemter, E, Lengger, C, Maier, H, Matloka, M, Möller, G, Naton, B, Prehn, C, Puk, O, Rácz, I, Rathkolb, B, Römisch-Margl, W, Rozman, J, Wang-Sattler, R, Schrewe, A, Stöger, C, Tost, M, Adamski, J, Aigner, B, Beckers, J, Behrendt, H, Busch, DH, Esposito, I, Graw, J, Illig, T, Ivandic, B, Klingenspor, M, Klopstock, T, Kremmer, E, Mempel, M, Neschen, S, Ollert, M, Schulz, H, Suhre, K, Wolf, E, Wurst, W, Zimmer, A & Hrabě de Angelis, M 2011, 'Mouse phenotyping', Methods, vol. 53, no. 2, pp. 120-135. https://doi.org/10.1016/j.ymeth.2010.08.006

TY - JOUR

T1 - Mouse phenotyping

AU - Fuchs, Helmut

AU - Gailus-Durner, Valérie

AU - Adler, Thure

AU - Aguilar-Pimentel, Juan Antonio

AU - Becker, Lore

AU - Calzada-Wack, Julia

AU - Da Silva-Buttkus, Patricia

AU - Neff, Frauke

AU - Götz, Alexander

AU - Hans, Wolfgang

AU - Hölter, Sabine M.

AU - Horsch, Marion

AU - Kastenmüller, Gabi

AU - Kemter, Elisabeth

AU - Lengger, Christoph

AU - Maier, Holger

AU - Matloka, Mikolaj

AU - Möller, Gabriele

AU - Naton, Beatrix

AU - Prehn, Cornelia

AU - Puk, Oliver

AU - Rácz, Ildikó

AU - Rathkolb, Birgit

AU - Römisch-Margl, Werner

AU - Rozman, Jan

AU - Wang-Sattler, Rui

AU - Schrewe, Anja

AU - Stöger, Claudia

AU - Tost, Monica

AU - Adamski, Jerzy

AU - Aigner, Bernhard

AU - Beckers, Johannes

AU - Behrendt, Heidrun

AU - Busch, Dirk H.

AU - Esposito, Irene

AU - Graw, Jochen

AU - Illig, Thomas

AU - Ivandic, Boris

AU - Klingenspor, Martin

AU - Klopstock, Thomas

AU - Kremmer, Elisabeth

AU - Mempel, Martin

AU - Neschen, Susanne

AU - Ollert, Markus

AU - Schulz, Holger

AU - Suhre, Karsten

AU - Wolf, Eckhard

AU - Wurst, Wolfgang

AU - Zimmer, Andreas

AU - Hrabě de Angelis, Martin

N1 - Funding Information: We would like to thank Reinhard Seeliger, Miriam Backs, Nicole Boche, Sabrina Bothur, Ralf Fischer, Michaela Grandl, Tamara Halex, Sabine Holthaus, Elfi Holupirek, Constanze König, Maria Kugler, Albert Langer, Katrin Laube, Astrid Markert, Jacqueline Müller, Elenore Samson, Sandra Schädler, Florian Schleicher, Daniela Schmidt, Waldemar Schneider, Ann-Elisabeth Schwarz, Anett Seelig, Bettina Sperling, Waltraud Stettinger, Lucie Thurmann, Susanne Wittich, Anja Wohlbier and Claudia Zeller as well as the GMC animal caretaker team for expert technical help. This work has been funded by the BMBF (NGFNplus Grant, 01GS08156 , 01GS0850 , 01GS0851 , 01GS0852 , 01GS0868 , 01GS0869 , 01GS0853 , and 01GS0854 ) and by an EU grant (EUMODIC, LSHG-2006-037188 , German Mouse Clinic).

PY - 2011/2

Y1 - 2011/2

N2 - Model organisms like the mouse are important tools to learn more about gene function in man. Within the last 20. years many mutant mouse lines have been generated by different methods such as ENU mutagenesis, constitutive and conditional knock-out approaches, knock-down, introduction of human genes, and knock-in techniques, thus creating models which mimic human conditions. Due to pleiotropic effects, one gene may have different functions in different organ systems or time points during development. Therefore mutant mouse lines have to be phenotyped comprehensively in a highly standardized manner to enable the detection of phenotypes which might otherwise remain hidden. The German Mouse Clinic (GMC) has been established at the Helmholtz Zentrum München as a phenotyping platform with open access to the scientific community (www.mousclinic.de; [1]). The GMC is a member of the EUMODIC consortium which created the European standard workflow EMPReSSslim for the systemic phenotyping of mouse models (http://www.eumodic.org/ [2]).

AB - Model organisms like the mouse are important tools to learn more about gene function in man. Within the last 20. years many mutant mouse lines have been generated by different methods such as ENU mutagenesis, constitutive and conditional knock-out approaches, knock-down, introduction of human genes, and knock-in techniques, thus creating models which mimic human conditions. Due to pleiotropic effects, one gene may have different functions in different organ systems or time points during development. Therefore mutant mouse lines have to be phenotyped comprehensively in a highly standardized manner to enable the detection of phenotypes which might otherwise remain hidden. The German Mouse Clinic (GMC) has been established at the Helmholtz Zentrum München as a phenotyping platform with open access to the scientific community (www.mousclinic.de; [1]). The GMC is a member of the EUMODIC consortium which created the European standard workflow EMPReSSslim for the systemic phenotyping of mouse models (http://www.eumodic.org/ [2]).

UR - http://www.scopus.com/inward/record.url?scp=79251597943&partnerID=8YFLogxK

U2 - 10.1016/j.ymeth.2010.08.006

DO - 10.1016/j.ymeth.2010.08.006

M3 - Review article

C2 - 20708688

AN - SCOPUS:79251597943

SN - 1046-2023

VL - 53

SP - 120

EP - 135

JO - Methods

JF - Methods

IS - 2

ER -

Mouse phenotyping

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