With increasing global concerns on food biosafety and security, there is increasing pressure on the accuracy of phytosanitary tests to discriminate potential pests and pathogens. The general demise of a classical taxonomic skill-base compounds these pressures further. Consequently, molecular diagnostic methodologies are being developed. To fulfil their function, molecular diagnostics designed to discriminate target organisms that could be utilised in phytosanitary/quarantine laboratories should be robust, simple and sensitive and thus validated rigorously before being implemented. In a nematological context, the majority of putative molecular diagnostics that have been developed to date target primarily either potato cyst nematode (PCN) or root knot nematode (RKN) species. Few have been developed for free-living virus-vector species. Here we review the published diagnostics for virus-vector nematode species and describe in detail a validation protocol that we argue should be utilised as a basis for all future nematode diagnostics.
|Title of host publication||Techniques in Diagnosis of Plant Viruses|
|Editors||G.P. Rao, R.A. Valverde, C.I. Dovas|
|Place of Publication||Houston|
|Publication status||Published - 2008|
|Name||Plant Pathogens Series|
|Publisher||Studium Press LLC|