Modulation of carcinogen bioavailability by immunisation with benzo[a]pyrene-conjugate vaccines

Nathalie Grova, Emmanuel J.F. Prodhomme, Mario T. Schellenberger, Sophie Farinelle, Claude P. Muller*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Abstract

Benzo[a]pyrene (B[a]P) conjugate vaccines based on ovalbumin, tetanus toxoid and diphtheria toxoid (DT) as carrier proteins were developed to investigate the effect of specific antibodies on the bioavailability of this ubiquitous carcinogen and its metabolites. After metabolic activation of this prototype carcinogen, B[a]P forms DNA adducts which initiate chemical carcinogenesis. B[a]P-DT conjugate induced the most robust immune response. The antibodies reacted not only with B[a]P but also with the proximate carcinogen 7,8-diol-B[a]P. Antibodies modulated the bioavailability of B[a]P and its metabolic activation in a dose-dependent manner by sequestration in the blood. Our results showed that this immune prophylactic strategy influences the pharmacokinetic of B[a]P and further studies to investigate their effects on chemical carcinogenesis are warranted.

Original languageEnglish
Pages (from-to)4142-4151
Number of pages10
JournalVaccine
Volume27
Issue number31
DOIs
Publication statusPublished - 24 Jun 2009

Keywords

  • Benzo[a]pyrene
  • Bioavailability
  • Mice
  • Specific antibodies

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