TY - JOUR
T1 - miR-512-3p as a Potential Biomarker of Poor Outcome in Pediatric Medulloblastoma
AU - Corrêa, Carolina Alves Pereira
AU - Chagas, Pablo Shimaoka
AU - Baroni, Mirella
AU - Andrade, Augusto Faria
AU - de Paula Queiroz, Rosane Gomes
AU - Suazo, Veridiana Kiill
AU - Veiga Cruzeiro, Gustavo Alencastro
AU - Fedatto, Paola Fernanda
AU - Antonio, David Santos Marco
AU - Brandalise, Silvia Regina
AU - Yunes, José Andres
AU - Panepucci, Rodrigo Alexandre
AU - Carlotti Junior, Carlos Gilberto
AU - de Oliveira, Ricardo Santos
AU - Neder, Luciano
AU - Tone, Luiz Gonzaga
AU - Valera, Elvis Terci
AU - Scrideli, Carlos Alberto
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025/6
Y1 - 2025/6
N2 - The tumorigenesis of medulloblastoma (MB), the most frequent malignant brain tumor in children, is not completely known. MicroRNA (miRNA) expression profiles have been associated with human cancers; however, the role played by miRNAs in pediatric MB has been poorly explored. Global miRNA expression in MB and non-neoplastic cerebellum samples was evaluated by microarray assay. Nine miRNAs (miR-31-5p, -329, -383, -433, -485-3p, -485-5p, -491, -512-3p, and 539-5p) in 51 pediatric MB and 7 pediatric non-neoplastic cerebellum samples were chosen for validation by qRT-PCR. The validated miRNAs were less expressed in the MB samples than in the non-neoplastic controls. In our cohort of patients, higher miR-512-3p expression was associated with incomplete degree of resection, classification as high risk, classification as group 4, and poor overall survival. In silico analysis in an independent cohort of MB patients identified that some of the miR-512-3p target genes were also correlated with prognostic features. Our results have shown that miR-512-3p could be associated with poor clinical outcomes in pediatric MB, suggesting that miR-512-3p is a potential biomarker of prognosis.
AB - The tumorigenesis of medulloblastoma (MB), the most frequent malignant brain tumor in children, is not completely known. MicroRNA (miRNA) expression profiles have been associated with human cancers; however, the role played by miRNAs in pediatric MB has been poorly explored. Global miRNA expression in MB and non-neoplastic cerebellum samples was evaluated by microarray assay. Nine miRNAs (miR-31-5p, -329, -383, -433, -485-3p, -485-5p, -491, -512-3p, and 539-5p) in 51 pediatric MB and 7 pediatric non-neoplastic cerebellum samples were chosen for validation by qRT-PCR. The validated miRNAs were less expressed in the MB samples than in the non-neoplastic controls. In our cohort of patients, higher miR-512-3p expression was associated with incomplete degree of resection, classification as high risk, classification as group 4, and poor overall survival. In silico analysis in an independent cohort of MB patients identified that some of the miR-512-3p target genes were also correlated with prognostic features. Our results have shown that miR-512-3p could be associated with poor clinical outcomes in pediatric MB, suggesting that miR-512-3p is a potential biomarker of prognosis.
KW - Cerebellum
KW - Medulloblastoma
KW - MicroRNA Profile
KW - miR-512-3p
UR - http://www.scopus.com/inward/record.url?scp=105000748578&partnerID=8YFLogxK
U2 - 10.1007/s12311-025-01812-3
DO - 10.1007/s12311-025-01812-3
M3 - Article
AN - SCOPUS:105000748578
SN - 1473-4222
VL - 24
JO - Cerebellum
JF - Cerebellum
IS - 3
M1 - 72
ER -