Minocycline hydrochloride as a potential adjuvant to improve osteoconductive and osteoinductive properties of bone substitutes in an extra-skeletal bone augmentation model: Preliminary observations in rats: Eur Dent Res Biomater J

Bob Biewer, Dorien Van Hede, Eric Rompen, Michel Mittelbronn, Pascale Quatresooz, Felix B Kleine Borgmann

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Objectives The present study was performed to determine if minocycline HCl could influence the behavior of deproteinized bovine bone mineral (DBBM) and bioactive glass (BG) particles when used as filler material for new bone generation in a guided bone augmentation model. Materials and Methods Two occlusive titanium caps were placed on the rat calvaria. One filled with BG particles, the second with DBBM particles, both previously mixed with blood (control). In minocycline HCl loaded groups (experimental), grafts were additionally placed into a minocycline solution. Samples were harvested after 4, 8, and 16 weeks. Half of the samples were embedded in methylmethacrylate for undecalcified histology and the other half was fixed, decalcified, and embedded in paraffin for classical histologic analysis. Results The control groups highlighted osteoconductive and osteoinductive responses associated to BG particles, as well as an osteoconductive reaction, in DBBM sections. The addition of minocycline HCl to BG particles had no measurable influence on the result. In minocycline HCl loaded DBBM sections; however, areas of spontaneous ossification could be observed after 8 and 16 weeks. Conclusion Our observations suggest that minocycline HCl may add some osteoinductive properties to DBBM within the limitations of this study design. Further investigations are needed to refine the present results.
Original languageUndefined/Unknown
JournalEuropean Dental Research and Biomaterials Journal
Publication statusPublished - 11 Jun 2021


  • bioactive glass, deproteinized bovine bone mineral, minocycline, hydrochloride, guided bone augmentation, extraskeletal bone formation, bone histology

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