Polycyclic aromatic hydrocarbons (PAHs) are lipophilic organic pollutants occurring widely in the terrestrial environment. To study the transfer of PAHs in the food chain, pigs have been fed with milk spiked either with [14C]phenanthrene or with [14C]benzo[α]pyrene. The analysis of blood radioactivity showed that both PAHs were absorbed with a maximum concentration at 5-6 h after milk ingestion, similar to fat metabolism. The blood radioactivity then decreased to reach background levels 24 h after milk ingestion. Furthermore, the blood radioactivity was higher for phenanthrene (even if the injected load was the lowest) than for benzo[α]pyrene, in agreement with their solubility difference. These findings suggest that milk fat and PAHs were absorbed during the same time period.