MicroRNA-149-3p expression correlates with outcomes of adrenocortical tumor patients and affects proliferation and cell cycle progression of H295A adrenocortical cancer cell line

Keteryne Rodrigues da Silva, Luciana Chain Veronez, Carolina Alves Pereira Correa, Régia Caroline Peixoto Lira, Mirella Baroni, Rosane de Paula Silva Queiroz, Sonir Roberto Rauber Antonini, José Andres Yunes, Silvia Regina Brandalise, Luiz Gonzaga Tone, Carlos Alberto Scrideli*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

Pediatric adrenocortical tumor (ACT) is a rare and aggressive neoplasm, with incidence in southern and southeastern Brazil 10–15 times higher than worldwide. Although microRNAs (miRNAs) have been reported to act as tumor suppressors or oncogenes in several cancers, the role of miR-149-3p in ACT remains unknown. In this study, we evaluated the expression of miR-149-3p in 67 pediatric ACT samples and 19 non-neoplastic adrenal tissues. The overexpression of miR-149-3p was induced in H295A cell line, and cell viability, proliferation, colony formation, and cell cycle were assessed by in miR-149-3p mimic or mimic control. In silico analysis were used to predict miR-149-3p putative target genes. CDKN1A expression at the mRNA and protein levels was evaluated by qRT-PCR and western blot, respectively. Higher miR-149-3p expression was associated with unfavorable ACT outcomes. Compared to the mimic control, miR-149-3p overexpression increased cell viability and colony formation, and affected cell cycle progression. Also, we identified CDKN1A as a potential miR-149-3p target gene, with decreased expression at both the gene and protein levels in miR-149-3p mimic cells.

Original languageEnglish
Pages (from-to)1952-1960
Number of pages9
JournalHuman Cell
Volume35
Issue number6
DOIs
Publication statusPublished - Nov 2022
Externally publishedYes

Keywords

  • Adrenocortical tumors
  • CDKN1A
  • Cell cycle
  • Cell viability
  • microRNA
  • miR-149-3p

Fingerprint

Dive into the research topics of 'MicroRNA-149-3p expression correlates with outcomes of adrenocortical tumor patients and affects proliferation and cell cycle progression of H295A adrenocortical cancer cell line'. Together they form a unique fingerprint.

Cite this