@inbook{94d94cbe63b4445da59dbe5cfdea175c,
title = "Metaproteomics: Sample Preparation and Methodological Considerations",
abstract = "Meta-omic techniques have progressed rapidly in the past decade and are frequently used in microbial ecology to study microorganisms in their natural ecosystems independent from culture restrictions. Metaproteomics, in combination with metagenomics, enables quantitative assessment of expressed proteins and pathways from individual members of the consortium. Together, metaproteomics and metagenomics can provide a detailed understanding of which organisms occupy specific metabolic niches, how they interact, and how they utilize nutrients, and these insights can be obtained directly from environmental samples. Here, we outline key aspects of sample preparation, database generation, and other methodological considerations that are required for successful quantitative metaproteomic analyses and we describe case studies on the integration with metagenomics for enhanced functional output.",
keywords = "Assembly, Binning, Functional analysis, Metagenomics, Metaproteomics, Microbial communities",
author = "Kunath, \{Benoit J.\} and Giusi Minniti and Morten Skaugen and Hagen, \{Live H.\} and Gustav Vaaje-Kolstad and Eijsink, \{Vincent G.H.\} and Pope, \{Phil B.\} and Magnus Arntzen",
note = "Publisher Copyright: {\textcopyright} 2019, Springer Nature Switzerland AG.",
year = "2019",
doi = "10.1007/978-3-030-12298-0\_8",
language = "English",
series = "Advances in Experimental Medicine and Biology",
publisher = "Springer New York LLC",
pages = "187--215",
booktitle = "Advances in Experimental Medicine and Biology",
}