MERTK-Dependent Ensheathment of Photoreceptor Outer Segments by Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

Seba Almedawar; Katerina Vafia; Sven Schreiter; Katrin Neumann; Shahryar Khattak; Thomas Kurth; Marius Ader; Mike O. Karl; Stephen H. Tsang; Elly M. Tanaka

doi:10.1016/j.stemcr.2020.02.004

MERTK-Dependent Ensheathment of Photoreceptor Outer Segments by Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

Seba Almedawar^*, Katerina Vafia, Sven Schreiter, Katrin Neumann, Shahryar Khattak, Thomas Kurth, Marius Ader, Mike O. Karl, Stephen H. Tsang, Elly M. Tanaka

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

18 Citations (Scopus)

Abstract

Using ultrastructural scanning electron microscopy (SEM)-based analysis, Almedawar et al. show that MERTK ligands, GAS6 and PROS1, upregulate photoreceptor outer segments (POS) ensheathment in human embryonic stem cell-derived retinal pigment epithelial cells. The authors also demonstrate that MERTK is essential for POS ensheathment and fragmentation before internalization, and suggest that ensheathment defects contribute to the pathology of retinitis pigmentosa type 38.

Original language	English
Pages (from-to)	374-389
Number of pages	16
Journal	Stem Cell Reports
Volume	14
Issue number	3
DOIs	https://doi.org/10.1016/j.stemcr.2020.02.004
Publication status	Published - 10 Mar 2020
Externally published	Yes

Keywords

GAS6
MERTK
MFGE8
PROS1
ensheathment
human embryonic stem cells
human pluripotent stem cells
phagocytosis
photoreceptor outer segments
retinal pigment epithelium

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10.1016/j.stemcr.2020.02.004

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title = "MERTK-Dependent Ensheathment of Photoreceptor Outer Segments by Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium",

abstract = "Using ultrastructural scanning electron microscopy (SEM)-based analysis, Almedawar et al. show that MERTK ligands, GAS6 and PROS1, upregulate photoreceptor outer segments (POS) ensheathment in human embryonic stem cell-derived retinal pigment epithelial cells. The authors also demonstrate that MERTK is essential for POS ensheathment and fragmentation before internalization, and suggest that ensheathment defects contribute to the pathology of retinitis pigmentosa type 38.",

keywords = "GAS6, MERTK, MFGE8, PROS1, ensheathment, human embryonic stem cells, human pluripotent stem cells, phagocytosis, photoreceptor outer segments, retinal pigment epithelium",

author = "Seba Almedawar and Katerina Vafia and Sven Schreiter and Katrin Neumann and Shahryar Khattak and Thomas Kurth and Marius Ader and Karl, {Mike O.} and Tsang, {Stephen H.} and Tanaka, {Elly M.}",

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year = "2020",

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day = "10",

doi = "10.1016/j.stemcr.2020.02.004",

language = "English",

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AU - Almedawar, Seba

AU - Vafia, Katerina

AU - Schreiter, Sven

AU - Neumann, Katrin

AU - Khattak, Shahryar

AU - Kurth, Thomas

AU - Ader, Marius

AU - Karl, Mike O.

AU - Tsang, Stephen H.

AU - Tanaka, Elly M.

PY - 2020/3/10

Y1 - 2020/3/10

N2 - Using ultrastructural scanning electron microscopy (SEM)-based analysis, Almedawar et al. show that MERTK ligands, GAS6 and PROS1, upregulate photoreceptor outer segments (POS) ensheathment in human embryonic stem cell-derived retinal pigment epithelial cells. The authors also demonstrate that MERTK is essential for POS ensheathment and fragmentation before internalization, and suggest that ensheathment defects contribute to the pathology of retinitis pigmentosa type 38.

AB - Using ultrastructural scanning electron microscopy (SEM)-based analysis, Almedawar et al. show that MERTK ligands, GAS6 and PROS1, upregulate photoreceptor outer segments (POS) ensheathment in human embryonic stem cell-derived retinal pigment epithelial cells. The authors also demonstrate that MERTK is essential for POS ensheathment and fragmentation before internalization, and suggest that ensheathment defects contribute to the pathology of retinitis pigmentosa type 38.

KW - GAS6

KW - MERTK

KW - MFGE8

KW - PROS1

KW - ensheathment

KW - human embryonic stem cells

KW - human pluripotent stem cells

KW - phagocytosis

KW - photoreceptor outer segments

KW - retinal pigment epithelium

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U2 - 10.1016/j.stemcr.2020.02.004

DO - 10.1016/j.stemcr.2020.02.004

M3 - Article

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AN - SCOPUS:85081643637

SN - 2213-6711

VL - 14

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JO - Stem Cell Reports

JF - Stem Cell Reports

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ER -

MERTK-Dependent Ensheathment of Photoreceptor Outer Segments by Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

Abstract

Keywords

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