TY - JOUR
T1 - MEIS homeodomain proteins facilitate PARP1/ ARTD1-mediated eviction of histone H1
AU - Hau, Ann Christin
AU - Grebbin, Britta Moyo
AU - Agoston, Zsuzsa
AU - Anders-Maurer, Marie
AU - Müller, Tamara
AU - Groß, Anja
AU - Kolb, Jasmine
AU - Langer, Julian D.
AU - Döring, Claudia
AU - Schulte, Dorothea
N1 - Funding Information:
The work was supported by the Schram Foundation (T287/ 21795/2011) and the Deutsche Forschungsgemeinschaft grant SCHU1218/3-1 to D. Schulte. The authors declare no competing financial interests.
Publisher Copyright:
© 2017 Hau et al.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Pre-B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal differentiation of adult subventricular zone-derived stem/progenitor cells, we describe a sequence of events by which PBX-MEIS facilitates chromatin accessibility of transcriptionally inactive genes: In undifferentiated cells, PBX1 is bound to the H1-compacted promoter/proximal enhancer of the neuron- specific gene doublecortin (Dcx). Once differentiation is induced, MEIS associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. These results for the first time link MEIS proteins to PARP-regulated chromatin dynamics and provide a mechanistic basis to explain the profound cellular changes elicited by these proteins.
AB - Pre-B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal differentiation of adult subventricular zone-derived stem/progenitor cells, we describe a sequence of events by which PBX-MEIS facilitates chromatin accessibility of transcriptionally inactive genes: In undifferentiated cells, PBX1 is bound to the H1-compacted promoter/proximal enhancer of the neuron- specific gene doublecortin (Dcx). Once differentiation is induced, MEIS associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. These results for the first time link MEIS proteins to PARP-regulated chromatin dynamics and provide a mechanistic basis to explain the profound cellular changes elicited by these proteins.
UR - http://www.scopus.com/inward/record.url?scp=85028825602&partnerID=8YFLogxK
U2 - 10.1083/jcb.201701154
DO - 10.1083/jcb.201701154
M3 - Article
C2 - 28739678
AN - SCOPUS:85028825602
SN - 0021-9525
VL - 216
SP - 2715
EP - 2729
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 9
ER -