TY - JOUR
T1 - Measuring CTL Lytic Granule Secretion and Target Cell Membrane Repair by Fluorescent Lipophilic Dye Uptake at the Lytic Synapse
AU - Müller, Sabina
AU - Filali, Liza
AU - Puissegur, Marie Pierre
AU - Valitutti, Salvatore
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023/4/28
Y1 - 2023/4/28
N2 - CD8+ cytotoxic T lymphocytes (CTL) play a key role in anti-tumor immune response. They are therefore at the heart of current immunotherapy protocols against cancer. Despite current strategies to potentiate CTL responses, cancer cells can resist CTL attack, thus limiting the efficacy of immunotherapies. To optimize immunotherapy, it is urgent to develop rapid assays allowing to assess CTL-cancer cell confrontation at the lytic synapse. In this chapter, we describe a flow cytometry-based method to simultaneously assess the extent of CTL activation and of tumor cell reparative membrane turnover in CTL/target cell conjugates. Such a method can be performed using a limited number of cells. It can therefore be employed in clinical settings when only a few patient-derived cells might be available.
AB - CD8+ cytotoxic T lymphocytes (CTL) play a key role in anti-tumor immune response. They are therefore at the heart of current immunotherapy protocols against cancer. Despite current strategies to potentiate CTL responses, cancer cells can resist CTL attack, thus limiting the efficacy of immunotherapies. To optimize immunotherapy, it is urgent to develop rapid assays allowing to assess CTL-cancer cell confrontation at the lytic synapse. In this chapter, we describe a flow cytometry-based method to simultaneously assess the extent of CTL activation and of tumor cell reparative membrane turnover in CTL/target cell conjugates. Such a method can be performed using a limited number of cells. It can therefore be employed in clinical settings when only a few patient-derived cells might be available.
KW - Cytotoxic T lymphocytes
KW - Flow cytometry
KW - Fluorescent lipophilic dyes
KW - Lytic synapse
KW - Plasma membrane turnover
KW - Time lapse video microscopy
UR - http://www.scopus.com/inward/record.url?scp=85158138543&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/37106201
U2 - 10.1007/978-1-0716-3135-5_30
DO - 10.1007/978-1-0716-3135-5_30
M3 - Article
C2 - 37106201
AN - SCOPUS:85158138543
SN - 1064-3745
VL - 2654
SP - 463
EP - 476
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -