Malarial hemozoin is a Nalp3 inflammasome activating danger signal

Catherine Dostert*, Greta Guarda, Jackeline F. Romero, Philippe Menu, Olaf Gross, Aubry Tardivel, Mario Luca Suva, Jean Christophe Stehle, Manfred Kopf, Ivan Stamenkovic, Giampietro Corradin, Jurg Tschopp

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

307 Citations (Scopus)


Background: Characteristic symptoms of malaria include recurrent fever attacks and neurodegeneration, signs that are also found in patients with a hyperactive Nalp3 inflammasome. Plasmodium species produce a crystal called hemozoin that is generated by detoxification of heme after hemoglobin degradation in infected red blood cells. Thus, we hypothesized that hemozoin could activate the Nalp3 inflammasome, due to its particulate nature reminiscent of other inflammasome-activating agents. Methodology/Principal Findings: We found that hemozoin acts as a proinflammatory danger signal that activates the Nalp3 inflammasome, causing the release of IL-1β. Similar to other Nalp3-activating particles, hemozoin activity is blocked by inhibiting phagocytosis, K+ efflux and NADPH oxidase. In vivo, intraperitoneal injection of hemozoin results in acute peritonitis, which is impaired in Nalp3-, caspase-1- and IL-1R-deficient mice. Likewise, the pathogenesis of cerebral malaria is dampened in Nalp3-deficient mice infected with Plasmodium berghei sporozoites, while parasitemia remains unchanged. Significance/Conclusions: The potent pro-inflammatory effect of hemozoin through inflammasome activation may possibly be implicated in plasmodium-associated pathologies such as cerebral malaria.

Original languageEnglish
Article numbere6510
JournalPLoS ONE
Issue number8
Publication statusPublished - 4 Aug 2009
Externally publishedYes


Dive into the research topics of 'Malarial hemozoin is a Nalp3 inflammasome activating danger signal'. Together they form a unique fingerprint.

Cite this