Lytic enzyme-based selective detection of Staphylococcus epidermidis using a porous silicon optical bacteria sensor

Jonathan Rasson*, Catherine Nannan, Jacques Mahillon, Laurent A. Francis

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this work, we developed a porous silicon optical bacteria sensor and demonstrated its use for a label-free and selective detection of Staphylococcus epidermidis bacteria. The selective optical detection of bacteria is explored by using a selectivity scheme based on the lytic enzyme lysostaphin to selectively target S. epidermidis instead of using a surface functionalization with antibodies. The detection is performed on a porous silicon sensor, passivated by atomic layer deposition of oxide for an improved stability in aqueous media, using Reflective Interferometric Fourier Transform Spectroscopy.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages573-574
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020
Externally publishedYes
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 22 Oct 201726 Oct 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period22/10/1726/10/17

Keywords

  • Bacteria sensor
  • Lytic enzyme
  • Optical sensor
  • Porous silicon

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