Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training

Ana Vujovic; Vesna Spasojevic-Kalimanovska; Natasa Bogavac-Stanojevic; Jelena Kotur-Stevuljevic; Miron Sopic; Aleksandra Stefanovic; Ivana Baralic; Brizita Djordjevic; Zorana Jelic-Ivanovic; Slavica Spasic

doi:10.5504/BBEQ.2012.0144

Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training

Ana Vujovic^*
, Vesna Spasojevic-Kalimanovska
, Natasa Bogavac-Stanojevic
, Jelena Kotur-Stevuljevic
, Miron Sopic
, Aleksandra Stefanovic
, Ivana Baralic
, Brizita Djordjevic
, Zorana Jelic-Ivanovic
, Slavica Spasic

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O₂), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O₂ concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, although they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period. The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of MDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O₂and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.

Original language	English
Pages (from-to)	3843-3847
Number of pages	5
Journal	Biotechnology and Biotechnological Equipment
Volume	27
Issue number	3
DOIs	https://doi.org/10.5504/BBEQ.2012.0144
Publication status	Published - Jan 2013
Externally published	Yes

Keywords

Cu/Zn SOD
Gene expression
Mn SOD
Oxidative stress
Soccer training

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10.5504/BBEQ.2012.0144

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Vujovic, A., Spasojevic-Kalimanovska, V., Bogavac-Stanojevic, N., Kotur-Stevuljevic, J., Sopic, M., Stefanovic, A., Baralic, I., Djordjevic, B., Jelic-Ivanovic, Z., & Spasic, S. (2013). Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training. Biotechnology and Biotechnological Equipment, 27(3), 3843-3847. https://doi.org/10.5504/BBEQ.2012.0144

@article{e8e6966293e941b8b0c2918df27a147e,

title = "Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training",

abstract = "The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O2), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O2 concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, although they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period. The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of MDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O2and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.",

keywords = "Cu/Zn SOD, Gene expression, Mn SOD, Oxidative stress, Soccer training",

author = "Ana Vujovic and Vesna Spasojevic-Kalimanovska and Natasa Bogavac-Stanojevic and Jelena Kotur-Stevuljevic and Miron Sopic and Aleksandra Stefanovic and Ivana Baralic and Brizita Djordjevic and Zorana Jelic-Ivanovic and Slavica Spasic",

year = "2013",

month = jan,

doi = "10.5504/BBEQ.2012.0144",

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Vujovic, A, Spasojevic-Kalimanovska, V, Bogavac-Stanojevic, N, Kotur-Stevuljevic, J, Sopic, M, Stefanovic, A, Baralic, I, Djordjevic, B, Jelic-Ivanovic, Z & Spasic, S 2013, 'Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training', Biotechnology and Biotechnological Equipment, vol. 27, no. 3, pp. 3843-3847. https://doi.org/10.5504/BBEQ.2012.0144

TY - JOUR

T1 - Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training

AU - Vujovic, Ana

AU - Spasojevic-Kalimanovska, Vesna

AU - Bogavac-Stanojevic, Natasa

AU - Kotur-Stevuljevic, Jelena

AU - Sopic, Miron

AU - Stefanovic, Aleksandra

AU - Baralic, Ivana

AU - Djordjevic, Brizita

AU - Jelic-Ivanovic, Zorana

AU - Spasic, Slavica

PY - 2013/1

Y1 - 2013/1

N2 - The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O2), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O2 concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, although they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period. The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of MDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O2and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.

AB - The purpose of the present study was to examine the effect of physical training on the gene expression levels of Cu/Zn superoxide dismutase (Cu/Zn SOD) and Mn superoxide dismutase (Mn SOD) in young soccer players at the beginning of their professional careers. The non-trained young men underwent a professional soccer training program of 12 weeks. Blood was obtained at two time points: before and after the 12-week endurance soccer training. Plasmatic superoxide dismutase (SOD) activity, concentration of sulfhydryl (SH) groups, superoxide anion (O2), malondialdehyde (MDA), and advanced oxidation protein product (AOPP) were determined. Messenger RNA (mRNA) relative levels of SOD isoenzymes were measured in peripheral blood mononuclear cells by Real-time PCR. After 12-week training, the soccer group experienced a significant decrease in plasmatic SOD activity and increase of O2 concentration. Concentration of SH groups, MDA and AOPP were not affected by physical training, although they showed a trend to increase. Cu/Zn SOD mRNA expression levels were stable during the endurance training period but Mn SOD mRNA levels were significantly higher after the training period. The variation in Mn SOD mRNA levels was dependent on MDA concentration. Negative prediction of MDA concentration on Mn SOD mRNA expression levels remained significant after inclusion of all the other oxidative stress status parameters in a multiple regression model (p = 0.017). Positive prediction of Cu/Zn SOD mRNA levels on plasmatic SOD activity remained significant after inclusion of MDA, log O2and AOPP concentrations in a multiple regression model (p = 0.025). Intensive endurance soccer training stimulated a beneficial increase in lymphocyte Mn SOD rather than in Cu/Zn SOD mRNA expression levels. An interaction between the oxidative stress/antioxidative defence status parameters in blood and gene expression levels of Cu/Zn SOD and Mn SOD in PBMCs was also observed.

KW - Cu/Zn SOD

KW - Gene expression

KW - Mn SOD

KW - Oxidative stress

KW - Soccer training

UR - https://www.scopus.com/pages/publications/84883257146

U2 - 10.5504/BBEQ.2012.0144

DO - 10.5504/BBEQ.2012.0144

M3 - Article

AN - SCOPUS:84883257146

SN - 1310-2818

VL - 27

SP - 3843

EP - 3847

JO - Biotechnology and Biotechnological Equipment

JF - Biotechnology and Biotechnological Equipment

IS - 3

ER -

Lymphocyte cu/znsod and mnsod gene expression responses to intensive endurance soccer training

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Keywords

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