Lck promotes Zap70-dependent LAT phosphorylation by bridging Zap70 to LAT

Wan Lin Lo, Neel H. Shah, Nagib Ahsan, Veronika Horkova, Ondrej Stepanek, Arthur R. Salomon, John Kuriyan, Arthur Weiss*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review


T cell-antigen receptor (TCR) signaling requires the sequential activities of the kinases Lck and Zap70. Upon TCR stimulation, Lck phosphorylates the TCR, thus leading to the recruitment, phosphorylation, and activation of Zap70. Lck binds and stabilizes phosho-Zap70 by using its SH2 domain, and Zap70 phosphorylates the critical adaptors LAT and SLP76, which coordinate downstream signaling. It is unclear whether phosphorylation of these adaptors occurs through passive diffusion or active recruitment. We report the discovery of a conserved proline-rich motif in LAT that mediates efficient LAT phosphorylation. Lck associates with this motif via its SH3 domain, and with phospho-Zap70 via its SH2 domain, thereby acting as a molecular bridge that facilitates the colocalization of Zap70 and LAT. Elimination of this proline-rich motif compromises TCR signaling and T cell development. These results demonstrate the remarkable multifunctionality of Lck, wherein each of its domains has evolved to orchestrate a distinct step in TCR signaling.

Original languageEnglish
Pages (from-to)733-741
Number of pages9
JournalNature Immunology
Issue number7
Publication statusPublished - 1 Jul 2018
Externally publishedYes


Dive into the research topics of 'Lck promotes Zap70-dependent LAT phosphorylation by bridging Zap70 to LAT'. Together they form a unique fingerprint.

Cite this