Laminin expression by glial fibrillary acidic protein positive cells in human gliomas

Berit BØlge Tysnes*, Rupavathana Mahesparan, Frits Thorsen, Hans Kristian Haugland, Torsten Porwol, Per Øyvind Enger, Morten Lund-Johansen, Rolf Bjerkvig

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

31 Citations (Scopus)


Extracellular matrix components are regarded as important substrates for invasive tumor cells. The present work focuses on the expression of laminin in the brain in response to invading brain tumors. Biopsies obtained from tissue macroscopically evaluated as the border zone between tumor and normal brain, in 5 patients undergoing surgery for glioblastoma multiforme, were examined by immunocytochemistry and scanning confocal microscopy for the expression of laminin and glial fibrillary acidic protein. Laminin was mainly found in all the specimens associated with the basal lamina of blood vessels, but a variable degree of punctate laminin deposits were also observed in the parenchyma not associated with blood vessels. In the specimens with substantial deposits, scanning confocal microscopy showed that some of the laminin co-localized with intracellular glial fibrillary acidic protein.Punctate deposits of laminin were also seen in an intracranial BT4C rat glioma model, where it was particularly abundant in the brain/tumor confrontation zone. Previous in vitro studies have shown that laminin, among several extracellular matrix components, represent a highly permissive substrate for glioma cell migration. The presented results indicate that laminin can be produced by glial fibrillary acidic protein positive cells during glioma cell invasion in humans. This glycoprotein may thus represent one important substrate among many, which contribute to the invasive phenotype of gliomas. Copyright (C) 1999 ISDN.

Original languageEnglish
Pages (from-to)531-539
Number of pages9
JournalInternational Journal of Developmental Neuroscience
Issue number5-6
Publication statusPublished - Aug 1999
Externally publishedYes


  • Glial fibrillary acidic protein
  • Glioblastoma
  • Invasion
  • Laminin


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