Lactate monitoring in organotypic 3D cell cultures

S. Hammer; A. Weltin; Y. Kaminski; F. Noor; J. Kieninger; G. A. Urban

doi:10.1016/j.proeng.2015.08.825

Lactate monitoring in organotypic 3D cell cultures

S. Hammer, A. Weltin^*, Y. Kaminski, F. Noor, J. Kieninger, G. A. Urban

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

4 Citations (Scopus)

Abstract

We present an electrochemical metabolic monitoring system, which is combined with a spheroid 3D tumor cell culture environment. It allows the continuous and precise monitoring of lactate production rates of single human hepatocyte spheroids online over days. Lactate concentration was measured using an electrochemical microsensor enabling the real-time monitoring of metabolic rates in combination with drug exposure. This setup can be used for drug testing as a powerful tool for fundamental research in general biology and toxicology.

Original language	English
Pages (from-to)	961-964
Number of pages	4
Journal	Procedia Engineering
Volume	120
DOIs	https://doi.org/10.1016/j.proeng.2015.08.825
Publication status	Published - 2015
Externally published	Yes
Event	29th European Conference on Solid-State Transducers, EUROSENSORS 2015; Freiburg; Germany; 6 September 2015 through 9 September 2015. - Freiburg, Germany Duration: 6 Sept 2015 → 9 Sept 2015

Keywords

3D cell culture
Cancer
Electrochemical
Lactate
Microsensors
Monitoring
Spheroids
Toxicology

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10.1016/j.proeng.2015.08.825

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title = "Lactate monitoring in organotypic 3D cell cultures",

abstract = "We present an electrochemical metabolic monitoring system, which is combined with a spheroid 3D tumor cell culture environment. It allows the continuous and precise monitoring of lactate production rates of single human hepatocyte spheroids online over days. Lactate concentration was measured using an electrochemical microsensor enabling the real-time monitoring of metabolic rates in combination with drug exposure. This setup can be used for drug testing as a powerful tool for fundamental research in general biology and toxicology.",

keywords = "3D cell culture, Cancer, Electrochemical, Lactate, Microsensors, Monitoring, Spheroids, Toxicology",

author = "S. Hammer and A. Weltin and Y. Kaminski and F. Noor and J. Kieninger and Urban, {G. A.}",

note = "Publisher Copyright: {\textcopyright} 2015 The Authors. Published by Elsevier Ltd.; 29th European Conference on Solid-State Transducers, EUROSENSORS 2015; Freiburg; Germany; 6 September 2015 through 9 September 2015. ; Conference date: 06-09-2015 Through 09-09-2015",

year = "2015",

doi = "10.1016/j.proeng.2015.08.825",

language = "English",

volume = "120",

pages = "961--964",

journal = "Procedia Engineering",

issn = "1877-7058",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - Lactate monitoring in organotypic 3D cell cultures

AU - Hammer, S.

AU - Weltin, A.

AU - Kaminski, Y.

AU - Noor, F.

AU - Kieninger, J.

AU - Urban, G. A.

PY - 2015

Y1 - 2015

N2 - We present an electrochemical metabolic monitoring system, which is combined with a spheroid 3D tumor cell culture environment. It allows the continuous and precise monitoring of lactate production rates of single human hepatocyte spheroids online over days. Lactate concentration was measured using an electrochemical microsensor enabling the real-time monitoring of metabolic rates in combination with drug exposure. This setup can be used for drug testing as a powerful tool for fundamental research in general biology and toxicology.

AB - We present an electrochemical metabolic monitoring system, which is combined with a spheroid 3D tumor cell culture environment. It allows the continuous and precise monitoring of lactate production rates of single human hepatocyte spheroids online over days. Lactate concentration was measured using an electrochemical microsensor enabling the real-time monitoring of metabolic rates in combination with drug exposure. This setup can be used for drug testing as a powerful tool for fundamental research in general biology and toxicology.

KW - 3D cell culture

KW - Cancer

KW - Electrochemical

KW - Lactate

KW - Microsensors

KW - Monitoring

KW - Spheroids

KW - Toxicology

UR - http://www.scopus.com/inward/record.url?scp=84985021293&partnerID=8YFLogxK

U2 - 10.1016/j.proeng.2015.08.825

DO - 10.1016/j.proeng.2015.08.825

M3 - Conference article

AN - SCOPUS:84985021293

SN - 1877-7058

VL - 120

SP - 961

EP - 964

JO - Procedia Engineering

JF - Procedia Engineering

T2 - 29th European Conference on Solid-State Transducers, EUROSENSORS 2015; Freiburg; Germany; 6 September 2015 through 9 September 2015.

Y2 - 6 September 2015 through 9 September 2015

ER -

Lactate monitoring in organotypic 3D cell cultures

Abstract

Keywords

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