Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation

Silvia Cristofanon; Francesco Uguccioni; Claudia Cerella; Flavia Radogna; Mario Dicato; Lina Ghibelli; Marc Diederich

doi:10.1111/j.1749-6632.2009.04896.x

Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation

Silvia Cristofanon^*, Francesco Uguccioni, Claudia Cerella, Flavia Radogna, Mario Dicato, Lina Ghibelli, Marc Diederich

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

52 Citations (Scopus)

Abstract

We have shown that melatonin exerts a prooxidant activity in U937 cells, a tumor human promonocytic cell line.¹ Here we show that melatonin induces a strong canonical activation of NF-κB, inducing IκBα degradation and the consequential nuclear translocation of p50/p65 subunits. The timing of NF-κB activation overlaps with the timing of reactive oxygen species (ROS) production due to melatonin. Overexpression of dominant-negative IκB, which prevents a possible NF-κB activation, transformed melatonin in a proapoptotic molecule. These data indicate for the first time that melatonin can trigger NF-κB activation and might suggest a possible role for ROS induced by melatonin. Results indicate a possible involvement in the survival pathway of melatonin-generated ROS as secondary messengers.

Original language	English
Title of host publication	Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways
Publisher	Blackwell Publishing Inc.
Pages	472-478
Number of pages	7
ISBN (Print)	9781573317375
DOIs	https://doi.org/10.1111/j.1749-6632.2009.04896.x
Publication status	Published - Aug 2009
Externally published	Yes

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1171
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

Melatonin
NF-κB
Reactive oxygen species
Survival

Access to Document

10.1111/j.1749-6632.2009.04896.x

Cite this

Cristofanon, S., Uguccioni, F., Cerella, C., Radogna, F., Dicato, M., Ghibelli, L., & Diederich, M. (2009). Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways (pp. 472-478). (Annals of the New York Academy of Sciences; Vol. 1171). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.04896.x

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title = "Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation",

abstract = "We have shown that melatonin exerts a prooxidant activity in U937 cells, a tumor human promonocytic cell line.1 Here we show that melatonin induces a strong canonical activation of NF-κB, inducing IκBα degradation and the consequential nuclear translocation of p50/p65 subunits. The timing of NF-κB activation overlaps with the timing of reactive oxygen species (ROS) production due to melatonin. Overexpression of dominant-negative IκB, which prevents a possible NF-κB activation, transformed melatonin in a proapoptotic molecule. These data indicate for the first time that melatonin can trigger NF-κB activation and might suggest a possible role for ROS induced by melatonin. Results indicate a possible involvement in the survival pathway of melatonin-generated ROS as secondary messengers.",

keywords = "Melatonin, NF-κB, Reactive oxygen species, Survival",

author = "Silvia Cristofanon and Francesco Uguccioni and Claudia Cerella and Flavia Radogna and Mario Dicato and Lina Ghibelli and Marc Diederich",

year = "2009",

month = aug,

doi = "10.1111/j.1749-6632.2009.04896.x",

language = "English",

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Cristofanon, S, Uguccioni, F, Cerella, C , Radogna, F, Dicato, M, Ghibelli, L & Diederich, M 2009, Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation. in Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Annals of the New York Academy of Sciences, vol. 1171, Blackwell Publishing Inc., pp. 472-478. https://doi.org/10.1111/j.1749-6632.2009.04896.x

Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation. / Cristofanon, Silvia; Uguccioni, Francesco; Cerella, Claudia et al.
Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc., 2009. p. 472-478 (Annals of the New York Academy of Sciences; Vol. 1171).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation

AU - Cristofanon, Silvia

AU - Uguccioni, Francesco

AU - Cerella, Claudia

AU - Radogna, Flavia

AU - Dicato, Mario

AU - Ghibelli, Lina

AU - Diederich, Marc

PY - 2009/8

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N2 - We have shown that melatonin exerts a prooxidant activity in U937 cells, a tumor human promonocytic cell line.1 Here we show that melatonin induces a strong canonical activation of NF-κB, inducing IκBα degradation and the consequential nuclear translocation of p50/p65 subunits. The timing of NF-κB activation overlaps with the timing of reactive oxygen species (ROS) production due to melatonin. Overexpression of dominant-negative IκB, which prevents a possible NF-κB activation, transformed melatonin in a proapoptotic molecule. These data indicate for the first time that melatonin can trigger NF-κB activation and might suggest a possible role for ROS induced by melatonin. Results indicate a possible involvement in the survival pathway of melatonin-generated ROS as secondary messengers.

AB - We have shown that melatonin exerts a prooxidant activity in U937 cells, a tumor human promonocytic cell line.1 Here we show that melatonin induces a strong canonical activation of NF-κB, inducing IκBα degradation and the consequential nuclear translocation of p50/p65 subunits. The timing of NF-κB activation overlaps with the timing of reactive oxygen species (ROS) production due to melatonin. Overexpression of dominant-negative IκB, which prevents a possible NF-κB activation, transformed melatonin in a proapoptotic molecule. These data indicate for the first time that melatonin can trigger NF-κB activation and might suggest a possible role for ROS induced by melatonin. Results indicate a possible involvement in the survival pathway of melatonin-generated ROS as secondary messengers.

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KW - Reactive oxygen species

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Cristofanon S, Uguccioni F, Cerella C , Radogna F, Dicato M, Ghibelli L et al. Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc. 2009. p. 472-478. (Annals of the New York Academy of Sciences). doi: 10.1111/j.1749-6632.2009.04896.x

Intracellular prooxidant activity of melatonin induces a survival pathway involving NF-κB activation

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