Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity

Maria Cristina Albertini; Flavia Radogna; Augusto Accorsi; Francesco Uguccioni; Laura Paternoster; Claudia Cerella; Milena De Nicola; Maria D'Alessio; Antonio Bergamaschi; Andrea Magrini; Lina Ghibelli

doi:10.1196/annals.1378.050

Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity

Maria Cristina Albertini^*, Flavia Radogna, Augusto Accorsi, Francesco Uguccioni, Laura Paternoster, Claudia Cerella, Milena De Nicola, Maria D'Alessio, Antonio Bergamaschi, Andrea Magrini, Lina Ghibelli

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

37 Citations (Scopus)

Abstract

It was long believed that melatonin might counteract intracellular oxidative stress because it was shown to potentiate antioxidant endogenous defences, and to increase the activity of many antioxidant enzymes. However, it is now becoming evident that when radicals are measured within cells, melatonin increases, rather than decreasing, radical production. Hereinwe demonstrate a pro-oxidant effect of melatonin in U937 cells by showing an increase of intracellular oxidative species and a depletion of glutathione (GSH). The activity of glutathione peroxidase is not modified by melatonin treatment as it does occur in other experimental models.

Original language	English
Title of host publication	Signal Transduction Pathways, Part B
Subtitle of host publication	Stress Signalling and Transcriptional Control
Publisher	Blackwell Publishing Inc.
Pages	10-16
Number of pages	7
ISBN (Print)	1573316474, 9781573316477
DOIs	https://doi.org/10.1196/annals.1378.050
Publication status	Published - Dec 2006
Externally published	Yes

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1091
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

Glutathione peroxidase
Melatonin
Pro-oxidant
Reduced glutathione
ROS

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10.1196/annals.1378.050

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Albertini, M. C., Radogna, F., Accorsi, A., Uguccioni, F., Paternoster, L., Cerella, C., De Nicola, M., D'Alessio, M., Bergamaschi, A., Magrini, A., & Ghibelli, L. (2006). Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity. In Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control (pp. 10-16). (Annals of the New York Academy of Sciences; Vol. 1091). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1378.050

Albertini, Maria Cristina ; Radogna, Flavia ; Accorsi, Augusto et al. / Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity. Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control. Blackwell Publishing Inc., 2006. pp. 10-16 (Annals of the New York Academy of Sciences).

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title = "Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity",

abstract = "It was long believed that melatonin might counteract intracellular oxidative stress because it was shown to potentiate antioxidant endogenous defences, and to increase the activity of many antioxidant enzymes. However, it is now becoming evident that when radicals are measured within cells, melatonin increases, rather than decreasing, radical production. Hereinwe demonstrate a pro-oxidant effect of melatonin in U937 cells by showing an increase of intracellular oxidative species and a depletion of glutathione (GSH). The activity of glutathione peroxidase is not modified by melatonin treatment as it does occur in other experimental models.",

keywords = "Glutathione peroxidase, Melatonin, Pro-oxidant, Reduced glutathione, ROS",

author = "Albertini, {Maria Cristina} and Flavia Radogna and Augusto Accorsi and Francesco Uguccioni and Laura Paternoster and Claudia Cerella and {De Nicola}, Milena and Maria D'Alessio and Antonio Bergamaschi and Andrea Magrini and Lina Ghibelli",

year = "2006",

month = dec,

doi = "10.1196/annals.1378.050",

language = "English",

isbn = "1573316474",

series = "Annals of the New York Academy of Sciences",

publisher = "Blackwell Publishing Inc.",

pages = "10--16",

booktitle = "Signal Transduction Pathways, Part B",

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Albertini, MC, Radogna, F, Accorsi, A, Uguccioni, F, Paternoster, L, Cerella, C, De Nicola, M, D'Alessio, M, Bergamaschi, A, Magrini, A & Ghibelli, L 2006, Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity. in Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control. Annals of the New York Academy of Sciences, vol. 1091, Blackwell Publishing Inc., pp. 10-16. https://doi.org/10.1196/annals.1378.050

Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity. / Albertini, Maria Cristina; Radogna, Flavia; Accorsi, Augusto et al.
Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control. Blackwell Publishing Inc., 2006. p. 10-16 (Annals of the New York Academy of Sciences; Vol. 1091).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity

AU - Albertini, Maria Cristina

AU - Radogna, Flavia

AU - Accorsi, Augusto

AU - Uguccioni, Francesco

AU - Paternoster, Laura

AU - Cerella, Claudia

AU - De Nicola, Milena

AU - D'Alessio, Maria

AU - Bergamaschi, Antonio

AU - Magrini, Andrea

AU - Ghibelli, Lina

PY - 2006/12

Y1 - 2006/12

N2 - It was long believed that melatonin might counteract intracellular oxidative stress because it was shown to potentiate antioxidant endogenous defences, and to increase the activity of many antioxidant enzymes. However, it is now becoming evident that when radicals are measured within cells, melatonin increases, rather than decreasing, radical production. Hereinwe demonstrate a pro-oxidant effect of melatonin in U937 cells by showing an increase of intracellular oxidative species and a depletion of glutathione (GSH). The activity of glutathione peroxidase is not modified by melatonin treatment as it does occur in other experimental models.

AB - It was long believed that melatonin might counteract intracellular oxidative stress because it was shown to potentiate antioxidant endogenous defences, and to increase the activity of many antioxidant enzymes. However, it is now becoming evident that when radicals are measured within cells, melatonin increases, rather than decreasing, radical production. Hereinwe demonstrate a pro-oxidant effect of melatonin in U937 cells by showing an increase of intracellular oxidative species and a depletion of glutathione (GSH). The activity of glutathione peroxidase is not modified by melatonin treatment as it does occur in other experimental models.

KW - Glutathione peroxidase

KW - Melatonin

KW - Pro-oxidant

KW - Reduced glutathione

KW - ROS

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U2 - 10.1196/annals.1378.050

DO - 10.1196/annals.1378.050

M3 - Conference contribution

C2 - 17341598

AN - SCOPUS:33947583289

SN - 1573316474

SN - 9781573316477

T3 - Annals of the New York Academy of Sciences

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BT - Signal Transduction Pathways, Part B

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ER -

Albertini MC, Radogna F, Accorsi A, Uguccioni F, Paternoster L, Cerella C et al. Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity. In Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control. Blackwell Publishing Inc. 2006. p. 10-16. (Annals of the New York Academy of Sciences). doi: 10.1196/annals.1378.050

Intracellular pro-oxidant activity of melatonin deprives U937 cells of reduced glutathione without affecting glutathione peroxidase activity

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