Intercellular transfer of transferrin receptor by a contact-, Rab8-dependent mechanism involving tunneling nanotubes

Anne Burtey*, Marek Wagner, Erlend Hodneland, Kai Ove Skaftnesmo, Julia Schoelermann, Ivan Rios Mondragon, Heidi Espedal, Anna Golebiewska, Simone P. Niclou, Rolf Bjerkvig, Tanja Kögel, Hans Hermann Gerdes

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

Intercellular communication between cancer cells, especially between cancer and stromal cells, plays an important role in disease progression. We examined the intercellular transfer of organelles and proteins in vitro and in vivo and the role of tunneling nanotubes (TNTs) in this process. TNTs are membrane bridges that facilitate intercellular transfer of organelles of unclear origin. Using 3-dimensional quantitative and qualitative confocal microscopy, we showed that TNTs contain green fluorescent protein (GFP)-early endosome antigen (EEA) 1, GFP Rab5, GFP Rab11, GFP Rab8, transferrin (Tf), and Tf receptor (Tf-R) fused to mCherry (Tf-RmCherry). Tf-Rm Cherry was transferred between cancer cells by a contactdependent but secretion-independent mechanism. Live cell imaging showed TNT formation preceding the transfer of Tf-Rm Cherry and involving the function of the small guanosine triphosphatase (GTPase) Rab8, which colocalized with Tf-Rm Cherry in the TNTs and was cotransferred to acceptor cells. Tf-Rm Cherry was transferred from cancer cells to fibroblasts, a noteworthy finding that suggests that this process occurs between tumor and stromal cells in vivo. We strengthened this hypothesis in a xenograft model of breast cancer using enhanced (e)GFP-expressing mice. Tf-Rm Cherry transferred from tumor to stromal cells and this process correlated with an increased opposite transfer of eGFP from stromal to tumor cells, together pointing toward complex intercellular communication at the tumor site.- Burtey, A., Wagner, M., Hodneland, E., Skaftnesmo, K. O., Schoelermann, J., Mondragon, I. R., Espedal, H., Golebiewska, A., Niclou, S. P., Bjerkvig, R., Kögel, T., Gerdes, H.-H. Intercellular transfer of transferrin receptor by a contact-, Rab8-dependent mechanism involving tunneling nanotubes.

Original languageEnglish
Pages (from-to)4695-4712
Number of pages18
JournalFASEB Journal
Volume29
Issue number11
DOIs
Publication statusPublished - 1 Nov 2015

Keywords

  • Cell-cell communication
  • Endosomes
  • Organelle exchange
  • Tumor microenvironment

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