TY - JOUR
T1 - Interaction between rat glioma cells and normal rat brain tissue in organ culture1, 2
AU - Steinsvåg, Sverre K.
AU - Laerum, Ole D.
AU - Bjerkvig, Rolf
N1 - Funding Information:
I Received July 10, 1984; accepted December 2, 1984. 2 Supported by the Norwegian Cancer Society. 3 Department of Pathology, The Gade Institute, University of Bergen, N-5016 Haukeland Hospital, Bergen, Norway. 4 Research fellow from the Norwegian Research Council for Sciences and the Humanities.
PY - 1985/5/1
Y1 - 1985/5/1
N2 - A system for coculture between normal rat brain fragments and multicellular spheroids of rat glioma cells is described. The tumor cells were derived from fetal BD IX rats treated transplacentally with the carcinogen N-ethyl-N-nitrosourea (CAS: 759-73-9). Brain fragments were obtained from fetal BD IX rats and precultured for 20 days before confrontation with multicellular tumor spheroids. The cocultures were grown in nonadherent stationary organ culture for 30 days. Due to morphologic similarities between normal brain cells and tumor cells, the tumor cells were labeled with tritiated thymidine, which made them easily recognizable in autoradiographs. The two structures adhered to each other, and glioma cells progressively invaded and replaced the normal brain tissue. Invasion and replacement are characteristic features of brain tumors in vivo. Therefore, this organotypic and syngeneic model may be useful for investigation of these phenomena outside the body.
AB - A system for coculture between normal rat brain fragments and multicellular spheroids of rat glioma cells is described. The tumor cells were derived from fetal BD IX rats treated transplacentally with the carcinogen N-ethyl-N-nitrosourea (CAS: 759-73-9). Brain fragments were obtained from fetal BD IX rats and precultured for 20 days before confrontation with multicellular tumor spheroids. The cocultures were grown in nonadherent stationary organ culture for 30 days. Due to morphologic similarities between normal brain cells and tumor cells, the tumor cells were labeled with tritiated thymidine, which made them easily recognizable in autoradiographs. The two structures adhered to each other, and glioma cells progressively invaded and replaced the normal brain tissue. Invasion and replacement are characteristic features of brain tumors in vivo. Therefore, this organotypic and syngeneic model may be useful for investigation of these phenomena outside the body.
UR - http://www.scopus.com/inward/record.url?scp=0021867601&partnerID=8YFLogxK
U2 - 10.1093/jnci/74.5.1095
DO - 10.1093/jnci/74.5.1095
M3 - Article
C2 - 3889456
AN - SCOPUS:0021867601
SN - 0027-8874
VL - 74
SP - 1095
EP - 1104
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 5
ER -