@article{ab50c68ffc2a4918b86218c71e23de56,
title = "Inhibition of medulloblastoma cell invasion by Slit",
abstract = "Invasion of brain tumor cells has made primary malignant brain neoplasms among the most recalcitrant to therapeutic strategies. We tested whether the secreted protein Slit2, which guides the projection of axons and developing neurons, could modulate brain tumor cell invasion. Slit2 inhibited the invasion of medulloblastoma cells in a variety of in vitro models. The effect of Slit2 was inhibited by the Robo ectodomain. Time-lapse videomicroscopy indicated that Slit2 reduced medulloblastoma invasion rate without affecting cell direction or proliferation. Both medulloblastoma and glioma tumors express Robo1 and Slit2, but only medulloblastoma invasion is inhibited by recombinant Slit2 protein. Downregulation of activated Cdc42 may contribute to this differential response. Our findings reinforce the concept that neurodevelopmental cues such as Slit2 may provide insights into brain tumor invasion.",
keywords = "Glioma, Inhibitor, Invasion, Medulloblastoma, Slit",
author = "Werbowetski-Ogilvie, {T. E.} and {Seyed Sadr}, M. and N. Jabado and A. Angers-Loustau and Agar, {N. Y.R.} and J. Wu and R. Bjerkvig and Antel, {J. P.} and D. Faury and Y. Rao and {Del Maestro}, {R. F.}",
note = "Funding Information: We thank Carmen Sabau, Roberta Waldkircher, and Samer Hussein for technical assistance (Montreal Neurological Institute, Montreal, Quebec). We also wish to thank Vinit Srivastava for providing the cerebellar tissue. TEWO is the recipient of a Canadian Institutes of Health Research (CIHR) Doctoral Research Award, MSS is the recipient of a Jeanne Timmins Costello Fellowship, NJ is a recipient of a Chercheur Boursier Salary award from Fonds de la Recherche en Sante du Quebec (FRSQ) and AAL is a recipient of the Terry Fox Studentship from the National Cancer Institute of Canada. RFDM holds the William Feindel Chair of Neurooncology and is a Killam Scholar at the Montreal Neurological Institute. This work was supported by the Goals for Lily and the Alex Pavanel Family, the Franco Di Giovanni, the Barbara Jacobson and Carol Cuthbertson, the Raymonde and Tony Boeckh Brain Tumor Research Funds, The Brain Tumour Foundation of Canada and the Maggie De Fontes Foundation.",
year = "2006",
month = aug,
day = "24",
doi = "10.1038/sj.onc.1209524",
language = "English",
volume = "25",
pages = "5103--5112",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Springer Nature",
number = "37",
}