Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks

Ophélie Fliniaux; Gwenaelle Gaillard; Antoine Lion; Dominique Cailleu; François Mesnard; Fotini Betsou

doi:10.1007/s10858-011-9574-5

Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks

Ophélie Fliniaux
, Gwenaelle Gaillard
, Antoine Lion
, Dominique Cailleu
, François Mesnard^*
, Fotini Betsou

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

51 Citations (Scopus)

Abstract

A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.

Original language	English
Pages (from-to)	457-465
Number of pages	9
Journal	Journal of Biomolecular NMR
Volume	51
Issue number	4
DOIs	https://doi.org/10.1007/s10858-011-9574-5
Publication status	Published - Dec 2011
Externally published	Yes

Keywords

Biobank
Biomarker
Biospecimen research
Metabolic profiling
Preanalytical variation
Proton NMR
Serum

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10.1007/s10858-011-9574-5

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title = "Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks",

abstract = "A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.",

keywords = "Biobank, Biomarker, Biospecimen research, Metabolic profiling, Preanalytical variation, Proton NMR, Serum",

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note = "Funding Information: Acknowledgments This work was supported by the Conseil R{\'e}gional de Picardie. We are grateful to Brian de Witt and Karsten Hiller for critical reading of the manuscript.",

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AU - Fliniaux, Ophélie

AU - Gaillard, Gwenaelle

AU - Lion, Antoine

AU - Cailleu, Dominique

AU - Mesnard, François

AU - Betsou, Fotini

N1 - Funding Information: Acknowledgments This work was supported by the Conseil Régional de Picardie. We are grateful to Brian de Witt and Karsten Hiller for critical reading of the manuscript.

PY - 2011/12

Y1 - 2011/12

N2 - A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.

AB - A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.

KW - Biobank

KW - Biomarker

KW - Biospecimen research

KW - Metabolic profiling

KW - Preanalytical variation

KW - Proton NMR

KW - Serum

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AN - SCOPUS:84855263249

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EP - 465

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

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ER -

Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks

Abstract

Keywords

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