TY - JOUR
T1 - Infection of rat brain cell aggregates with neurovirulent and nonneurovirulent strains of herpes simplex virus type 1
AU - Rødahl, Eyvind
AU - Pedersen, Paal Henning
AU - Bjerkvig, Rolf
AU - Haarr, Lars
N1 - Funding Information:
This work was supported by grants from the Norwegian Cancer Society and the Research Council of Norway. We are indebted to Drs. Jack G. Stevens, David C. Bloom, and Farhad Sedarati for providing RE6, R13-1, tk−7, and KD6. The technical assistance of Mrs. Kjerstin Jacobsen is greatly appreciated.
PY - 1999/4/10
Y1 - 1999/4/10
N2 - Rat brain cell aggregates represent a three-dimensional tissue culture system of brain tissue in the form of small, multicellular spheroids. In the present work, we have infected these 'minibrains' with neurovirulent, nonneurovirulent, and nonreplicating strains of HSV-1. The neurovirulent strains 17+ and KOS(M) spread rapidly through the aggregates, while the nonreplicating ICP4 deletion mutant KD6 infected cells only at the periphery of the aggregates. Spread and replication of the nonneurovirulent strains RE6 and tk-7, and to some extent also of R13/1, were restricted. The interaction between different strains of HSV-1 and the rat brain cell aggregates is thus comparable to that seen in the brain, suggesting that the aggregates represent a useful tool for studying HSV-1 infection of brain tissue in vitro.
AB - Rat brain cell aggregates represent a three-dimensional tissue culture system of brain tissue in the form of small, multicellular spheroids. In the present work, we have infected these 'minibrains' with neurovirulent, nonneurovirulent, and nonreplicating strains of HSV-1. The neurovirulent strains 17+ and KOS(M) spread rapidly through the aggregates, while the nonreplicating ICP4 deletion mutant KD6 infected cells only at the periphery of the aggregates. Spread and replication of the nonneurovirulent strains RE6 and tk-7, and to some extent also of R13/1, were restricted. The interaction between different strains of HSV-1 and the rat brain cell aggregates is thus comparable to that seen in the brain, suggesting that the aggregates represent a useful tool for studying HSV-1 infection of brain tissue in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0033541426&partnerID=8YFLogxK
U2 - 10.1006/excr.1998.4348
DO - 10.1006/excr.1998.4348
M3 - Article
C2 - 10094836
AN - SCOPUS:0033541426
SN - 0014-4827
VL - 248
SP - 306
EP - 313
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -