TY - JOUR
T1 - Increased [18F]FDG uptake in the infarcted myocardial area displayed by combined PET/CMR correlates with snRNA-seq-detected inflammatory cell invasion
AU - Lukovic, Dominika
AU - Gyöngyösi, Mariann
AU - Pavo, Imre J.
AU - Mester-Tonczar, Julia
AU - Einzinger, Patrick
AU - Zlabinger, Katrin
AU - Kastner, Nina
AU - Spannbauer, Andreas
AU - Traxler, Denise
AU - Pavo, Noemi
AU - Goliasch, Georg
AU - Pils, Dietmar
AU - Jakab, Andras
AU - Szankai, Zsuzsanna
AU - Michel-Behnke, Ina
AU - Zhang, Lu
AU - Devaux, Yvan
AU - Graf, Senta
AU - Beitzke, Dietrich
AU - Winkler, Johannes
N1 - Funding
Open access funding provided by Medical University of Vienna. This work is supported by COST (European Cooperation in Science and Technology) Action EU-CardioRNA CA17129. Yvan Devaux is the chair the COST Action.© 2024. The Author(s).
Publisher Copyright:
© The Author(s) 2024.
PY - 2024/6/26
Y1 - 2024/6/26
N2 - Combined [18F]FDG PET-cardiac MRI imaging (PET/CMR) is a useful tool to assess myocardial viability and cardiac function in patients with acute myocardial infarction (AMI). Here, we evaluated the prognostic value of PET/CMR in a porcine closed-chest reperfused AMI (rAMI) model. Late gadolinium enhancement by PET/CMR imaging displayed tracer uptake defect at the infarction site by 3 days after the rAMI in the majority of the animals (group Match, n = 28). Increased [18F]FDG uptake at the infarcted area (metabolism/contractility mismatch) with reduced tracer uptake in the remote viable myocardium (group Mismatch, n = 12) 3 days after rAMI was observed in the animals with larger infarct size and worse left ventricular ejection fraction (LVEF) (34 ± 8.7 vs 42.0 ± 5.2%), with lower LVEF also at the 1-month follow-up (35.8 ± 9.5 vs 43.0 ± 6.3%). Transcriptome analyses by bulk and single-nuclei RNA sequencing of the infarcted myocardium and border zones (n = 3 of each group, and 3 sham-operated controls) revealed a strong inflammatory response with infiltration of monocytes and macrophages in the infarcted and border areas in Mismatch animals. Our data indicate a high prognostic relevance of combined PET/MRI in the subacute phase of rAMI for subsequent impairment of heart function and underline the adverse effects of an excessive activation of the innate immune system in the initial phase after rAMI.
AB - Combined [18F]FDG PET-cardiac MRI imaging (PET/CMR) is a useful tool to assess myocardial viability and cardiac function in patients with acute myocardial infarction (AMI). Here, we evaluated the prognostic value of PET/CMR in a porcine closed-chest reperfused AMI (rAMI) model. Late gadolinium enhancement by PET/CMR imaging displayed tracer uptake defect at the infarction site by 3 days after the rAMI in the majority of the animals (group Match, n = 28). Increased [18F]FDG uptake at the infarcted area (metabolism/contractility mismatch) with reduced tracer uptake in the remote viable myocardium (group Mismatch, n = 12) 3 days after rAMI was observed in the animals with larger infarct size and worse left ventricular ejection fraction (LVEF) (34 ± 8.7 vs 42.0 ± 5.2%), with lower LVEF also at the 1-month follow-up (35.8 ± 9.5 vs 43.0 ± 6.3%). Transcriptome analyses by bulk and single-nuclei RNA sequencing of the infarcted myocardium and border zones (n = 3 of each group, and 3 sham-operated controls) revealed a strong inflammatory response with infiltration of monocytes and macrophages in the infarcted and border areas in Mismatch animals. Our data indicate a high prognostic relevance of combined PET/MRI in the subacute phase of rAMI for subsequent impairment of heart function and underline the adverse effects of an excessive activation of the innate immune system in the initial phase after rAMI.
KW - Acute myocardial infarction
KW - Cardiac PET–MRI imaging
KW - Innate immune response
KW - RNA sequencing
KW - [F]FDG
UR - http://www.scopus.com/inward/record.url?scp=85197316226&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/38922408
U2 - 10.1007/s00395-024-01064-y
DO - 10.1007/s00395-024-01064-y
M3 - Article
C2 - 38922408
AN - SCOPUS:85197316226
SN - 0300-8428
JO - Basic Research in Cardiology
JF - Basic Research in Cardiology
ER -