TY - JOUR
T1 - Increased CD45RA+CD45RO+ cells indicate activated T cells after endurance exercise
AU - Gabriel, Holger
AU - Schmitt, Barbara
AU - Urhausen, Axel
AU - Kindermann, Wilfried
PY - 1993/12
Y1 - 1993/12
N2 - Twelve endurance athletes performed either a 240-km cycle race in the Alps or an ultratriathlon (time: 731 ±25 min). Two weeks before (SI) and on the 8th or 9th day after (S2) the competition, an exhaustive exercise at 110% of the individual anaerobic threshold was performed (stress test; S1: 21 ± 2 min; S2: 18 ± 2 min). Blood samples at rest, at 10 min, at end, and 60 min after S1 or S2 were taken for immunophenotyping of leukocytes with fluorescein-or phycoerythrin-conjugated monoclonal antibodies (CD3, CD4, CD8, CD14, CD16, CD45, CD45RO, CD45RA, CD69, HLA-DR) and dual color flow cytometry. Of the lymphocyte subpopulations, the CD45RO+-lymphocytes had higher cell numbers at S2 than at S1 (P < 0.01). This was due to the increase of CD45RA +CD45RO+ lymphocytes (P < 0.005), but not to the CD45RA-CD45RO+ lymphocytes (P > 0.05). Both the CD4+ (+39%) and CD8+ (+75%) cells were increased at S2. The activation markers CD69 and HLA-DR were not significantly increased at S2. Our conclusion is that a ~12-h endurance exercise induces activation of T-cells, which is indicated by the increase of CD45RA +CD45RO+. This might also indicate an increase of memory cells by prolonged endurance exercise.
AB - Twelve endurance athletes performed either a 240-km cycle race in the Alps or an ultratriathlon (time: 731 ±25 min). Two weeks before (SI) and on the 8th or 9th day after (S2) the competition, an exhaustive exercise at 110% of the individual anaerobic threshold was performed (stress test; S1: 21 ± 2 min; S2: 18 ± 2 min). Blood samples at rest, at 10 min, at end, and 60 min after S1 or S2 were taken for immunophenotyping of leukocytes with fluorescein-or phycoerythrin-conjugated monoclonal antibodies (CD3, CD4, CD8, CD14, CD16, CD45, CD45RO, CD45RA, CD69, HLA-DR) and dual color flow cytometry. Of the lymphocyte subpopulations, the CD45RO+-lymphocytes had higher cell numbers at S2 than at S1 (P < 0.01). This was due to the increase of CD45RA +CD45RO+ lymphocytes (P < 0.005), but not to the CD45RA-CD45RO+ lymphocytes (P > 0.05). Both the CD4+ (+39%) and CD8+ (+75%) cells were increased at S2. The activation markers CD69 and HLA-DR were not significantly increased at S2. Our conclusion is that a ~12-h endurance exercise induces activation of T-cells, which is indicated by the increase of CD45RA +CD45RO+. This might also indicate an increase of memory cells by prolonged endurance exercise.
KW - CD45RA
KW - Cyclists
KW - Leukocyte common antigen
KW - Memory lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=0027486264&partnerID=8YFLogxK
U2 - 10.1249/00005768-199312000-00006
DO - 10.1249/00005768-199312000-00006
M3 - Article
C2 - 8107541
AN - SCOPUS:0027486264
SN - 0195-9131
VL - 25
SP - 1352
EP - 1357
JO - Medicine and Science in Sports and Exercise
JF - Medicine and Science in Sports and Exercise
IS - 12
ER -