TY - JOUR
T1 - Increased anti-inflammatory activity and enhanced phytochemical concentrations in superfine powders obtained by controlled differential sieving process from four medicinal plants
AU - Soulimani, Rachid
AU - Dicko, Amadou
AU - Baudelaire, Elie
AU - Bouayed, Jaouad
N1 - Publisher Copyright:
© 2021 Hogrefe AG.
PY - 2023/8
Y1 - 2023/8
N2 - Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100- 180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction. Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses. Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50- 100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05). For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts. Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.
AB - Anti-inflammatory effect of Rosa canina, Salix alba, Scrophularia nodosa and Hedera helix were studied in LPS-stimulated primary peripheral blood mononuclear cells (PBMCs) from mice (n=18) by comparing homogeneous powders of small microparticles (50-100 μm, 100- 180 μm and 180-315 μm) obtained from plants via a controlled differential sieving process (CDSp) versus total plant materials obtained via hydroethanolic (HE) extraction. Further, phytochemical composition of the fine powders and HE extracts was determined by LC-PDA-ESI/MS analyses. Results showed that a one-hour pretreatment of PBMCs with fine powders, particularly those with superfine particle sizes (i.e. 50- 100 μm and 100-180 μm), significantly inhibited TNFα, IL-1β, IL-6 and NO production in LPS-stimulated PBMCs, by at least ca. 20% more than HE extracts (all, p<0.05). For each of the plants studied, their superfine powdered fractions were more concentrated in phenolic contents than their HE extracts. Overall, our results further confirm CDSp, as an environmentally friendly method, for improving the concentration of bioactive compounds as well as their biological activities.
KW - Anti-inflammatory effect
KW - Controlled differential sieving process
KW - PBMCs
KW - Phytochemical concentrations
KW - Plant powders
UR - http://www.scopus.com/inward/record.url?scp=85120173328&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/34814709
U2 - 10.1024/0300-9831/a000739
DO - 10.1024/0300-9831/a000739
M3 - Article
C2 - 34814709
AN - SCOPUS:85120173328
SN - 0300-9831
VL - 93
SP - 339
EP - 351
JO - International Journal for Vitamin and Nutrition Research
JF - International Journal for Vitamin and Nutrition Research
IS - 4
ER -