In vivo multiphoton microscopy of deep tissue with gradient index lenses

Michael J. Levene; Daniel A. Dombeck; Rebecca M. Williams; Jesse Skoch; Gregory A. Hickey; Karl A. Kasischke; Raymond P. Molloy; Martin Ingelsson; Edward A. Stern; Jochen Klucken; Brian J. Bacskai; Warren R. Zipfel; Bradley T. Hyman; W. W. Webb

doi:10.1117/12.537285

In vivo multiphoton microscopy of deep tissue with gradient index lenses

Michael J. Levene^*, Daniel A. Dombeck, Rebecca M. Williams, Jesse Skoch, Gregory A. Hickey, Karl A. Kasischke, Raymond P. Molloy, Martin Ingelsson, Edward A. Stern, Jochen Klucken, Brian J. Bacskai, Warren R. Zipfel, Bradley T. Hyman, W. W. Webb

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

1 Citation (Scopus)

Abstract

Gradient index lenses enable multiphoton microscopy of deep tissues in the intact animal. In order to assess their applicability to clinical research, we present in vivo multiphoton microscopy with gradient index lenses in brain regions associated with Alzheimer's disease and Parkinson's disease in both transgenic and wild-type mice. We also demonstrate microscopy of ovary in wild type mouse using only intrinsic fluorescence and second harmonic generation, signal sources which may prove useful for both the study and diagnosis of cancer.

Original language	English
Pages (from-to)	291-296
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5323
DOIs	https://doi.org/10.1117/12.537285
Publication status	Published - 2004
Externally published	Yes
Event	Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV - San Jose, CA, United States Duration: 25 Jan 2004 → 27 Jan 2004

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Levene, M. J., Dombeck, D. A., Williams, R. M., Skoch, J., Hickey, G. A., Kasischke, K. A., Molloy, R. P., Ingelsson, M., Stern, E. A., Klucken, J., Bacskai, B. J., Zipfel, W. R., Hyman, B. T., & Webb, W. W. (2004). In vivo multiphoton microscopy of deep tissue with gradient index lenses. Proceedings of SPIE - The International Society for Optical Engineering, 5323, 291-296. https://doi.org/10.1117/12.537285

@article{0270002d16c844b3a8e2132a506ad5da,

title = "In vivo multiphoton microscopy of deep tissue with gradient index lenses",

abstract = "Gradient index lenses enable multiphoton microscopy of deep tissues in the intact animal. In order to assess their applicability to clinical research, we present in vivo multiphoton microscopy with gradient index lenses in brain regions associated with Alzheimer's disease and Parkinson's disease in both transgenic and wild-type mice. We also demonstrate microscopy of ovary in wild type mouse using only intrinsic fluorescence and second harmonic generation, signal sources which may prove useful for both the study and diagnosis of cancer.",

author = "Levene, {Michael J.} and Dombeck, {Daniel A.} and Williams, {Rebecca M.} and Jesse Skoch and Hickey, {Gregory A.} and Kasischke, {Karl A.} and Molloy, {Raymond P.} and Martin Ingelsson and Stern, {Edward A.} and Jochen Klucken and Bacskai, {Brian J.} and Zipfel, {Warren R.} and Hyman, {Bradley T.} and Webb, {W. W.}",

year = "2004",

doi = "10.1117/12.537285",

language = "English",

volume = "5323",

pages = "291--296",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV ; Conference date: 25-01-2004 Through 27-01-2004",

}

Levene, MJ, Dombeck, DA, Williams, RM, Skoch, J, Hickey, GA, Kasischke, KA, Molloy, RP, Ingelsson, M, Stern, EA, Klucken, J, Bacskai, BJ, Zipfel, WR, Hyman, BT & Webb, WW 2004, 'In vivo multiphoton microscopy of deep tissue with gradient index lenses', Proceedings of SPIE - The International Society for Optical Engineering, vol. 5323, pp. 291-296. https://doi.org/10.1117/12.537285

TY - JOUR

T1 - In vivo multiphoton microscopy of deep tissue with gradient index lenses

AU - Levene, Michael J.

AU - Dombeck, Daniel A.

AU - Williams, Rebecca M.

AU - Skoch, Jesse

AU - Hickey, Gregory A.

AU - Kasischke, Karl A.

AU - Molloy, Raymond P.

AU - Ingelsson, Martin

AU - Stern, Edward A.

AU - Klucken, Jochen

AU - Bacskai, Brian J.

AU - Zipfel, Warren R.

AU - Hyman, Bradley T.

AU - Webb, W. W.

PY - 2004

Y1 - 2004

N2 - Gradient index lenses enable multiphoton microscopy of deep tissues in the intact animal. In order to assess their applicability to clinical research, we present in vivo multiphoton microscopy with gradient index lenses in brain regions associated with Alzheimer's disease and Parkinson's disease in both transgenic and wild-type mice. We also demonstrate microscopy of ovary in wild type mouse using only intrinsic fluorescence and second harmonic generation, signal sources which may prove useful for both the study and diagnosis of cancer.

AB - Gradient index lenses enable multiphoton microscopy of deep tissues in the intact animal. In order to assess their applicability to clinical research, we present in vivo multiphoton microscopy with gradient index lenses in brain regions associated with Alzheimer's disease and Parkinson's disease in both transgenic and wild-type mice. We also demonstrate microscopy of ovary in wild type mouse using only intrinsic fluorescence and second harmonic generation, signal sources which may prove useful for both the study and diagnosis of cancer.

UR - http://www.scopus.com/inward/record.url?scp=5644299090&partnerID=8YFLogxK

U2 - 10.1117/12.537285

DO - 10.1117/12.537285

M3 - Conference article

AN - SCOPUS:5644299090

SN - 0277-786X

VL - 5323

SP - 291

EP - 296

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV

Y2 - 25 January 2004 through 27 January 2004

ER -

In vivo multiphoton microscopy of deep tissue with gradient index lenses

Abstract

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