In vitro and in vivo models for the study of brain tumour invasion

G. J. Pilkington*, R. Bjerkvig, L. De Ridder, P. Kaaijk

*Corresponding author for this work

Research output: Contribution to journalShort surveypeer-review

62 Citations (Scopus)


Since it is difficult to study the dynamic biological aspects of brain tumour invasion using histological sections of surgical biopsy and autopsy tissues, various laboratory systems have been devised. Animal models are less than ideal as chemically-induced brain tumours suffer from the fact that they have a low incidence and a long latency, while transplanted tumours grow predominantly by expansion, due to high proliferative activity, and not by diffuse local invasion as in human brain tumours. Various in vitro assays have, therefore, been established for both migration and invasion. These include the simple scratch technique in a confluent cell monolayer, the use of cloning rings and the 'Transwell' modified Boyden chamber technique. More complex three-dimensional culture model systems have also been developed, using chick heart optic nerve or reaggregated fetal brain as 'targets' for the invasion of neoplastic glia. Each method has yielded important information on the mechanisms which underlie brain tumour invasion. Moreover, individual microenvironmental factors may be modulated in these laboratory systems to determine their influence on the migration/invasion process.

Original languageEnglish
Pages (from-to)4107-4109
Number of pages3
JournalAnticancer Research
Issue number6 B
Publication statusPublished - Nov 1997
Externally publishedYes


  • Brain tumour
  • In vitro model
  • In vivo model
  • Invasion


Dive into the research topics of 'In vitro and in vivo models for the study of brain tumour invasion'. Together they form a unique fingerprint.

Cite this